S. Shrestha

Positive cooperativity enhances coupling of a second G protein to a GPCR dimer

The paper shows that a second G protein couples with higher affinity to a GPCR dimer than the first, due to conformational stabilization by the first G protein.

TOR signaling regulates GPCR levels on the plasma membrane and suppresses the Saccharomyces cerevisiae mating pathway

This study shows TOR regulates GPCR localization and signaling in yeast by controlling endocytosis and autophagy, suppressing the mating pathway.

Regenerative human liver organoids (HLOs) in a pillar/perfusion plate for hepatotoxicity assays

This paper develops a bioprinted liver organoid system for high-throughput hepatotoxicity assays, showing improved maturity, reproducibility, and regenerative capacity after drug exposure.

Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity

This study evaluates developmental neurotoxicity using dynamic cerebral organoid cultures in a pillar/perfusion plate, showing methylmercury induces changes in morphology and gene expression under dynamic conditions but not static ones.

Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting

This paper develops a microarray 3D bioprinting method to generate human liver organoids on a pillar plate, enabling high-throughput, reproducible hepatotoxicity assays with improved efficiency and function.

A resource to enable chemical biology and drug discovery of WDR Proteins

The paper develops a resource for discovering ligands for WDR proteins using experimental and computational methods, leading to the identification of drug-like ligands for 9 out of 20 targets.

Identification, antimicrobial resistance and molecular detection of Shiga toxin-producing E. coli (STEC) isolated from raw milk of cattle in Chitwan, Nepal

This study identifies and characterizes Shiga toxin-producing E. coli (STEC) in raw cattle milk from Nepal, finding a prevalence of 11.6% and highlighting antimicrobial resistance patterns.

Recruitment of FBXO22 for Targeted Degradation of NSD2

This paper describes a targeted protein degradation strategy using UNC8732 to recruit FBXO22 for NSD2 degradation, leading to growth suppression and reversal of drug resistance in leukemia cells.

Redox Regulation of Brain Selective Kinases BRSK1/2: Implications for Dynamic Control of the Eukaryotic AMPK family through Cys-based mechanisms

This study investigates how redox regulation via cysteine oxidation controls BRSK1/2 kinase activity, revealing conserved mechanisms that modulate catalytic function through disulfide bond formation and salt bridge disruption.

Caloric restriction promotes beta cell longevity and delays aging and senescence by enhancing cell identity and homeostasis mechanisms.

This study shows caloric restriction enhances beta cell identity and homeostasis, delays aging and senescence, and improves mitochondrial function in pancreatic beta cells.

Analyses of cell wall synthesis in Clostridioides difficile reveal a diversification in cell division mechanisms in endospore-forming bacteria

This study reveals that C. difficile uses a bifunctional class A penicillin-binding protein for cell division during normal growth, diverging from the typical FtsW-FtsI divisome mechanism.

The MODY-associated KCNK16 L114P mutation increases islet glucagon secretion and limits insulin secretion resulting in transient neonatal diabetes and glucose dyshomeostasis in adults

This study shows that the KCNK16 L114P mutation causes neonatal diabetes and glucose dyshomeostasis in adult mice by disrupting islet insulin secretion and increasing glucagon secretion.

Septin Organization is Regulated by the Gpa1 Ubiquitination Domain and Endocytic Machinery During the Yeast Pheromone Response.

This study shows that Gpa1 ubiquitination and endocytic machinery regulate septin organization during yeast pheromone response by controlling their localization at the polarity site.

Tyramine promotes colon cancer risk and development by inducing DNA damage and inflammation

This study shows tyramine increases colon cancer risk by causing DNA damage and inflammation in mice.

Simultaneous carbon catabolite repression governs sugar and aromatic co-utilization in Pseudomonas putida M2

This study investigates carbon catabolite repression in Pseudomonas putida M2 during simultaneous sugar and aromatic co-utilization, revealing simultaneous repression by sugars and aromatics and the role of crc and small RNAs.

Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate

This paper introduces a method to generate reproducible cerebral organoids from iPSCs using a pillar plate platform, improving throughput and reducing variability compared to traditional methods.

COMBINATION THERAPIES TARGETING ALK-ABERRANT NEUROBLASTOMA IN PRECLINICAL MODELS.

This study evaluates lorlatinib, an ALK inhibitor, in combination with chemotherapy or idasanutlin in preclinical neuroblastoma models, finding synergy in models with high ALK expression.

Mechanistic and evolutionary insights into isoform-specific 'supercharging' in DCLK family kinases

This study investigates how isoform-specific variations in DCLK family kinases affect autoregulation through structural and functional analyses.

A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis

This paper introduces a microarray 3D bioprinting platform with pillar and perfusion plates for high-throughput human organoid culture and analysis.

Simulating Rumen Conditions using an Anaerobic Dynamic Membrane Bioreactor to Enhance Hydrolysis of Lignocellulosic Biomass

This study uses an anaerobic dynamic membrane bioreactor to mimic rumen conditions, achieving high hydrolysis and VFA production from food waste.

A pillar/perfusion plate enhances cell growth, reproducibility, throughput, and user friendliness in dynamic 3D cell culture

The paper introduces a pillar/perfusion plate for dynamic 3D cell culture, improving growth, reproducibility, throughput, and user friendliness compared to static methods.

Single-spore germination analyses reveal that calcium released during Clostridioides difficile germination functions in a feed-forward loop.

This study uses single-spore germination analysis to show that Ca2+ is not essential for C. difficile spore germination but can enhance germination through a feedforward loop.

Evolutionary and cellular analysis of the dark pseudokinase PSKH2

This study investigates the evolutionary and cellular roles of the pseudokinase PSKH2, confirming its catalytic inactivity and identifying its mitochondrial localization and chaperone-dependent regulation.

Importin alpha/βeta Promote Kif18B Microtubule Association to Spatially Control Microtubule Destabilization

This study shows importin alpha/beta enhance Kif18B microtubule association, spatially controlling MT destabilization, while EB1 has a distinct role in this process.

RFX6-mediated dysregulation defines human β cell dysfunction in early type 2 diabetes

This study identifies RFX6 as a key regulator of β cell dysfunction in early type 2 diabetes through multi-omics analysis of human pancreatic tissue.

ETV2 regulates PARP-1 binding protein to induce ER stress-mediated cell death in tuberin-deficient cells

This study identifies ETV2 as a regulator of cell death in Tsc2-deficient cells through PARPBP and ER stress, suggesting a potential therapeutic target for LAM.

Evolution of the folding landscape of effector caspases

This study examines the folding landscape of human effector caspases and their common ancestor, showing that the folding mechanism was established early in evolution and retained for over 650 million years.

An Efficient and Cost-effective Purification Methodology for Cas9 Nuclease

This paper introduces a rapid, efficient, and cost-effective method to purify active SaCas9 protein with over 90% purity within 24 hours.

Case based learning as a dynamic approach towards learning oral pathology.

This study evaluates case-based learning's impact on dental students' understanding of oral pathology, finding significant improvements in knowledge and skills compared to conventional methods.

STUDY ON BREEDING PERFORMANCE, SURVIVAL AND GROWTH PERFORMANCE OF COMMON CARP (Cyprinus carpio L.) IN FISH FARM OF DHANUSHA DISTRICT, NEPAL

The study examines breeding performance, survival, and growth of common carp in Nepal's fish farms using induced breeding and monitoring water quality and growth metrics.