Author

E. Shifrut

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Immunology· 6 Bioengineering· 1

Signature Assays, Organisms & Methods

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Organism:human· 6 Assay:CRISPR screen· 5 Approach:experimental assay· 5 Research Focus:cell type annotation· 4 Research Focus:drug response / drug discovery· 3 Research Focus:gene regulatory network inference· 3 Approach:genetic perturbation / CRISPR· 3 Organism:mouse· 2

Frequent Co-Authors

A. Marson· 7 shared T. L. Roth· 4 shared A. Ashworth· 3 shared J. A. Bluestone· 3 shared J. Carnevale· 3 shared R. Apathy· 3 shared D. Goodman· 2 shared D. N. Nguyen· 2 shared

Activity by Year

2018-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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7 shared papersImmunology

A. Marson

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

Modular Pooled Discovery of Synthetic Knockin Sequences to Program Durable Cell Therapies

F. Blaeschke, Y. Y. Chen, R. Apathy, Z. Li, C. T. Mowery, W. A. Nyberg, A. To, R. Yu, R. Bueno, M. C. Kim, R. Schmidt, D. B. Goodman, T. Feuchtinger, J. Eyquem, C. J. Ye, , A. Marson

The paper develops ModPoKI, a platform for modular construction of DNA knockin libraries to identify gene constructs that enhance T cell function and persistence.

bioRxiv · Jul 27, 2022Immunology

Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence

J. Belk, W. Yao, N. Ly, K. Freitas, Y.-T. Chen, Q. Shi, A. Valencia, E. Shifrut, N. Kale, K. Yost, C. Duffy, M. Hwee, Z. Miao, A. Ashworth, C. Mackall, , A. Satpathy

This study identifies chromatin remodeling factors that limit T cell persistence using CRISPR screens and shows their impact on anti-tumor immunity.

bioRxiv · Apr 20, 2022Immunology

Hypoxia is a dominant remodeler of the CD8+ T cell surface proteome relative to activation and regulatory T cell-mediated suppression

J. R. Byrnes, A. M. Weeks, J. Carnevale, E. Shifrut, L. Kirkemo, A. Ashworth, A. Marson, J. A. Wells

This study compares how hypoxia and regulatory T cells affect the CD8+ T cell surface proteome, finding hypoxia has a more significant impact than Treg-mediated suppression.

bioRxiv · Jun 7, 2021Immunology

CRISPR Screen in Regulatory T Cells Reveals Ubiquitination Modulators of Foxp3

J. T. Cortez, E. Montauti, E. Shifrut, Y. Zhang, O. Shaked, Y. Xu, T. Roth, D. Simeonov, Y. Zhang, S. Chen, Z. Li, I. A. Vogel, G. Y. Prator, B. Zhang, Y. Lee, , D. Fang

This study uses a CRISPR screen in mouse Tregs to identify genes regulating Foxp3 expression, finding Usp22 and Atxn7l3 as stabilizers and Rnf20 as a destabilizer.

bioRxiv · Feb 26, 2020 · PreprintImmunology

Rapid discovery of synthetic DNA sequences to rewrite endogenous T cell circuits

T. L. Roth, P. J. Li, J. Nies, R. Yu, M. L. T. Nguyen, Y. Lee, R. Apathy, A. Truong, J. Hiatt, D. Wu, D. N. Nguyen, D. Goodman, J. A. Bluestone, K. Roybal, E. Shifrut, A. Marson

This paper develops a platform to discover synthetic DNA sequences that enhance T cell functions by integrating them into endogenous genomic sites.

bioRxiv · Apr 12, 2019 · PreprintImmunology

A Cas9 nanoparticle system with truncated Cas9 target sequences on DNA repair templates enhances genome targeting in diverse human immune cell types

D. N. Nguyen, T. L. Roth, J. Li, P. A. Chen, M. M. Mamedov, L. T. Vo, V. Tobin, R. Apathy, D. Goodman, E. Shifrut, J. A. Bluestone, J. M. Puck, F. C. Szoka, A. Marson

The paper improves non-viral CRISPR-Cas9 genome editing in immune cells by using truncated Cas9 target sequences and polymer-stabilized nanoparticles, enhancing efficiency and viability.

bioRxiv · Mar 28, 2019 · PreprintBioengineering

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function

E. Shifrut, J. Carnevale, V. Tobin, T. L. Roth, J. M. Woo, C. Bui, P. J. Li, M. Diolaiti, A. Ashworth, A. Marson

This study uses CRISPR screens in primary human T cells to identify regulators of immune function and cancer cell killing.

bioRxiv · Aug 3, 2018Immunology