G. A. Kwong

Spatiotemporal Control of Genomics and Epigenomics by Ultrasound

This paper develops FUS-CRISPR(a/i) tools to remotely control gene expression and epigenetic modifications in vivo, showing therapeutic effects in tumor models.

Embracing enzyme promiscuity with activity-based compressed biosensing

This paper introduces SLICE, a method to select promiscuous protease substrates for compressed sensing, enabling accurate classification of complex protease mixtures with fewer substrates.

Sensitizing solid tumors to CAR-mediated cytotoxicity using synthetic antigens

This study develops synthetic antigens to enhance CAR T cell efficacy against solid tumors by reducing off-target effects and improving tumor targeting.

In vivo mRNA delivery to virus-specific T cells by light-induced ligand exchange of MHC class I antigen-presenting nanoparticles

The paper develops a method to deliver mRNA to multiple virus-specific T cells using light-induced ligand exchange of MHC class I antigen-presenting nanoparticles, achieving ~50% transfection in vivo.

Synthetic Antigen-Presenting Cells for Adoptive T Cell Therapy

This paper develops synthetic antigen-presenting cells (APCs) that activate T cells effectively without conventional APCs, showing improved tumor control in mice.

Activity-based urinary biomarkers of response and resistance to checkpoint blockade immunotherapy

This paper develops INSIGHT, a mass-barcoded peptide sensor for noninvasive monitoring of checkpoint blockade therapy response and resistance in mice.

Remote control of CAR T cell therapies by thermal targeting

This paper develops a thermal targeting platform to remotely control CAR T cell therapies, showing significant enhancement in anti-tumor activity and survival in mouse models.

Prodrug defiance reveals logic-based strategies for treating bacterial resistance

This paper develops a model to classify bacterial defiance against prodrugs and introduces a dimensionless quantity to predict susceptibility or defiance.

Proteases as Biological Bits for Programmable Medicine

This paper programs protease activity in cell-free systems to perform classical and probabilistic algorithms for diagnosing pulmonary embolism in a mouse model.

Deconvolving multiplexed protease signatures with substrate reduction and activity clustering

The paper introduces a computational method to estimate protease activities from multiplexed substrate cleavage data by reducing substrates and clustering proteases into families.