Author

M. D. Leonetti

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Cell Biology· 7 Bioengineering· 2

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 6 Organism:human· 6 Approach:experimental assay· 5 Research Focus:cell signaling & trafficking· 4 Assay:proteomics· 4 Approach:microscopy & imaging analysis· 3 Research Focus:cancer & tumor microenvironment· 2 Research Focus:cell-cell communication· 2

Frequent Co-Authors

B. Huang· 3 shared M. Y. Hein· 3 shared P. Raghavan· 3 shared S. B. Mehta· 3 shared S. Bax· 3 shared C. Liu· 2 shared D. N. Itzhak· 2 shared D. R. Southworth· 2 shared

Activity by Year

2017-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 32 mutual co-author links

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3 shared papersCell Biology

B. Huang

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Author profile →

9 papers

Recent·Most cited

Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells

I. E. Ivanov, E. Hirata-Miyasaki, T. Chandler, R. C. Kovilakam, Z. Liu, S. Pradeep, C. Liu, M. Bhave, S. Khadka, C. Arias, M. D. Leonetti, B. Huang, S. B. Mehta

The paper introduces Mantis, a high-throughput 4D microscope, and shrimPy, software for analyzing 4D data, to study cellular dynamics and responses to perturbations like viral infection.

bioRxiv · Dec 19, 2023Cell Biology

Global organelle profiling reveals subcellular localization and remodeling at proteome scale

M. Y. Hein, D. Peng, V. Todorova, F. McCarthy, K. Kim, C. Liu, L. Savy, C. Januel, R. Baltazar-Nunez, S. Bax, S. Vaid, M. Vangipuram, I. E. Ivanov, J. R. Byrum, S. Pradeep, , M. D. Leonetti

The paper maps subcellular localization of human proteins and shows proteome remodeling during viral infection by combining organelle immuno-capture with mass spectrometry.

bioRxiv · Dec 18, 2023Cell Biology

An open-source FACS automation system for high-throughput cell biology

D. M. Wiener, E. Huynh, I. Jeyakumar, S. Bax, S. Sama, J. P. Cabrera, V. Todorova, M. Vangipuram, S. Vaid, F. Otsuka, Y. Sakai, M. D. Leonetti, R. Gomez-Sjoberg

The paper develops an open-source FACS automation system that reduces labor and errors in high-throughput cell sorting by integrating hardware and software for autonomous sample handling and gate generation.

bioRxiv · Mar 24, 2023Bioengineering

Oxaliplatin kills cells via liquid-liquid demixing of nucleoli

H. B. Schmidt, Z. A. Jaafar, J. J. Rodencal, M. D. Leonetti, S. J. Dixon, R. Rohatgi, O. Brandman

This study shows oxaliplatin causes nucleolar liquid-liquid demixing, leading to cell death by disrupting nucleolar organization and transcription.

bioRxiv · Jun 10, 2021 · PreprintCell Biology

WASP integrates substrate topology and cell polarity to guide neutrophil migration

R. M. Brunetti, G. Kockelkoren, P. Raghavan, G. R. R. Bell, D. Britain, N. Puri, S. R. Collins, M. D. Leonetti, D. Stamou, O. D. Weiner

This study shows WASP integrates substrate topology and cell polarity to guide neutrophil migration by selectively polymerizing actin at the leading edge.

bioRxiv · May 12, 2021Cell Biology

OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization

N. H. Cho, K. C. Cheveralls, A.-D. Brunner, K. Kim, A. C. Michaelis, P. Raghavan, H. Kobayashi, L. Savy, J. Y. Li, H. Canaj, J. Y. S. Kim, E. M. Setwart, C. Gnann, F. McCarthy, J. P. Cabrera, , M. D. Leonetti

The paper maps human protein localization and interactions using genome engineering, imaging, and data science, revealing functional communities and RNA-binding protein subgroups.

bioRxiv · Mar 29, 2021Cell Biology

PIKfyve inhibition blocks endolysosomal escape of α-synuclein fibrils and spread of α-synuclein aggregation

S. K. See, M. Chen, S. Bax, R. Tian, A. Woerman, E. Tse, I. E. Johnson, C. Nowotny, E. N. Munoz, J. Sengstack, D. R. Southworth, J. E. Gestwicki, M. D. Leonetti, M. Kampmann

The paper identifies PIKfyve inhibition as a strategy to block α-synuclein fibril escape from lysosomes, reducing aggregation spread in a human cell model.

bioRxiv · Jan 21, 2021 · PreprintCell Biology

Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation

J. J. Chen, D. L. Nathaniel, P. Raghavan, M. Nelson, R. Tian, E. Tse, J. Y. Hong, S. K. See, S.-A. Mok, M. Y. Hein, D. R. Southworth, L. T. Grinberg, J. E. Gestwicki, M. D. Leonetti, M. Kampmann

The paper identifies ESCRT pathway components as regulators of tau aggregation propagation by enabling endolysosomal escape of tau seeds.

bioRxiv · Sep 26, 2019Cell Biology

Improved Split Fluorescent Proteins For Endogenous Protein Labeling

S. Feng, S. Sekine, V. Pessino, H. Li, M. D. Leonetti, B. Huang

The paper develops improved split fluorescent proteins for endogenous protein labeling, showing enhanced signal-to-background ratios and new applications in super-resolution microscopy.

bioRxiv · Jul 26, 2017Bioengineering