R. Tian

Discovery of Alkenyl Oxindole as a Novel PROTAC Moiety for Targeted Protein Degradation via CRL4DCAF11 Recruitment

The paper develops JQ1-alkenyl oxindole conjugates that degrade BRD4 via the ubiquitin-proteasome system and recruits CRL4DCAF11, identifying HL435 as a potent antitumor lead.

CDC20 determines the sensitivity to spindle assembly checkpoint (SAC) inhibitors

The paper identifies CDC20 expression as a determinant of sensitivity to SAC inhibitors, showing that aneuploid cells with high CDC20 levels are more sensitive to these drugs.

RASP: Optimal single fluorescent puncta detection in complex cellular backgrounds

RASP detects fluorescent puncta in complex cellular backgrounds with high precision and speed, outperforming existing methods by using image gradients to separate objects from noise.

Chemical proteomic approach for in-depth glycosylation profiling of plasma carcinoembryonic antigen in cancer patients

This paper develops a chemical proteomic method to profile site-specific glycosylation of low-abundance plasma CEA in cancer patients, revealing differential glycoforms associated with cancer types and progression stages.

Sirenian genomes illuminate the evolution of fully aquatic species within the mammalian superorder Afrotheria

This study analyzes sirenian genomes to identify genomic adaptations to aquatic life and conservation challenges.

Structural basis for ssDNA-activated NADase activity of the prokaryotic SPARTA immune system

The paper determines the structure of the SPARTA immune system, revealing how it activates NADase activity in response to ssDNA targets.

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

The paper uses CRISPR screens in iPSC-derived neurons to identify factors controlling tau aggregation, finding new pathways and potential therapeutic targets for tauopathies.

A molecular glue approach to control the half-life of CRISPR-based technologies

This paper introduces a molecular glue approach using pomalidomide to control the half-life of CRISPR-based technologies, enabling precise regulation of their activity and therapeutic potential.

SunTag-PE: a modular prime editing system enables versatile and efficient genome editing

The paper introduces a modular prime editing system called SunTag-PE that improves delivery efficiency without compromising editing efficiency.

Evolutionary transcriptomics reveals longevity mostly driven by polygenic and indirect selection in mammals

This study uses comparative transcriptomics across mammals to show that longevity is influenced by polygenic and indirect selection, with key pathways like insulin signaling showing correlations with lifespan.

dATP Elevation Induces Myocardial Metabolic Remodeling to Support Improved Cardiac Function

This study shows that elevating dATP in cardiomyocytes improves cardiac function by enhancing contractility and metabolic flexibility through mitochondrial remodeling.

The Functional Landscape of SARS-CoV-2 3CL Protease

This study maps the functional landscape of SARS-CoV-2 3CL protease by profiling all single mutants and identifies resistance mutations to nirmatrelvir.

Regulation of protein translation by TRIM21

This study identifies TRIM21 as a regulator of PKR-mediated protein translation by modulating PP1 ubiquitination and eIF2 dephosphorylation, revealing its role in antiviral defense and potential therapeutic applications.

Regulation of the PKD2 channel function by TACAN

This study identifies TACAN as a PKD2 inhibitor that modulates its channel activity and mechano sensitivity in renal cells and zebrafish.

Selective inhibition reveals the regulatory function of DYRK2 in protein synthesis and calcium entry

This study develops a selective DYRK2 inhibitor, C17, and identifies novel targets like 4E-BP1 and STIM1, revealing DYRK2's role in protein synthesis and calcium entry.

PIKfyve inhibition blocks endolysosomal escape of α-synuclein fibrils and spread of α-synuclein aggregation

The paper identifies PIKfyve inhibition as a strategy to block α-synuclein fibril escape from lysosomes, reducing aggregation spread in a human cell model.

BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2

This study identifies BRD2 as a regulator of ACE2 transcription and shows that BRD2 inhibitors block SARS-CoV-2 infection in human cells and animal models.

Novel Three-Dimensional Organoid Sarcoidosis Granuloma Model

The paper develops a 3D organoid sarcoidosis model using patient-derived granulomas and microfluidics to study cytokine profiles in a lung-on-chip system.

A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling

The paper develops a CRISPR interference screen to identify regulators of GPCR/cAMP signaling, identifying 45 strong and 50 weaker regulators, and validates seven novel mediators.

Activity of the GHRH Antagonist MIA602 and its Underlying Mechanisms of Action in sarcoidosis

This study investigates the anti-inflammatory effects of MIA602, a GHRH antagonist, in sarcoidosis by analyzing its impact on cytokine levels and cellular markers in vitro and in vivo.

NAD+ Redox Imbalance in the Heart Exacerbates Diabetic Cardiomyopathy

This study shows that NAD+ redox imbalance worsens diabetic cardiomyopathy by impairing cardiomyocyte function and suggests NAMPT as a therapeutic target.

Structure Basis for Inhibition of SARS-CoV-2 by the Feline Drug GC376

The paper determines the structure of SARS-CoV-2 Mpro and shows how GC376 inhibits its catalytic site, supporting its use as an antiviral drug.

CRISPR-based screens uncover determinants of immunotherapy response in Multiple Myeloma

This study uses CRISPR screens to identify mechanisms increasing BCMA expression and enhancing anti-myeloma efficacy of BCMA-targeted therapies in multiple myeloma.

Lower Soil Carbon Loss Due to Persistent Microbial Adaptation to Climate Warming

This study shows that microbial adaptation reduces soil carbon loss under long-term warming by decreasing temperature sensitivity of respiration.

Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation

The paper identifies ESCRT pathway components as regulators of tau aggregation propagation by enabling endolysosomal escape of tau seeds.

Extraordinary diversity of HLA class I gene expression in single cells contribute to the plasticity and adaptability of human immune system

The paper uses scRNA-seq to show that single cells from the same donor can express different HLA class I genotypes, revealing high allele-specific expression diversity in the immune system.

Mitochondrial dysfunction is signaled to the integrated stress response by OMA1, DELE1 and HRI

The paper identifies OMA1, DELE1, and HRI as a pathway that relays mitochondrial dysfunction to the integrated stress response, showing its role in activating eIF2 kinase activity and potential therapeutic relevance.

CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons

This study develops a CRISPRi platform for genetic screens in human iPSC-derived neurons, identifying gene knockdown effects on survival, transcriptomics, and morphology.

Pan-genome analysis reveals the molecular basis of niche adaptation of Staphylococcus epidermidis strains

This study analyzes the pan-genome of Staphylococcus epidermidis to identify genomic factors associated with its adaptation to diverse niches and pathogenicity.

Contraction of the ROS scavenging enzyme glutathione S-transferase gene family in cetaceans

This study examines the evolution of the glutathione S-transferase gene family in cetaceans, finding lineage-specific gene losses and positive selection, suggesting adaptation to aquatic environments.