R. Rohatgi

Substrate-directed control of N-glycosylation in the endoplasmic reticulum calibrates signal reception at the cell-surface

The paper identifies an ER pathway that regulates N-glycosylation by modulating OST-A activity, impacting cell-surface signaling and tissue development.

Direct ionic stress sensing and mitigation by the transcription factor NFAT5

This study identifies NFAT5 as a direct sensor and effector of ionic stress in animal cells, showing it forms condensates to activate genes restoring ion balance.

The Energetics and Ion Coupling of Cholesterol Transport Through Patched1

The paper uses molecular dynamics simulations and biochemical assays to study cholesterol transport through PTCH1, finding energetic barriers and ion-coupling mechanisms for cholesterol export.

The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium

This study identifies the exocyst complex and Rab GTPases as key factors in the trafficking of soluble proteins, such as Gli transcription factors, to the primary cilium.

Oxaliplatin kills cells via liquid-liquid demixing of nucleoli

This study shows oxaliplatin causes nucleolar liquid-liquid demixing, leading to cell death by disrupting nucleolar organization and transcription.

Sphingomyelin suppresses Hedgehog signaling by restricting cholesterol accessibility at the ciliary membrane

This study identifies sphingomyelin's role in restricting cholesterol accessibility at the ciliary membrane, thereby suppressing Hedgehog signaling.

TDP-43 α-helical structure tunes liquid-liquid phase separation and function

This study shows that mutations in TDP-43's helical region modulate its phase separation and function, with implications for ALS and synthetic biology.

Decoding and recoding phase behavior of TDP43 reveals that phase separation is not required for splicing function

The paper shows that TDP43's splicing function does not require phase separation, using mutagenesis and live-cell assays to analyze phase dynamics.

Bile Acid Biosynthesis Avoiding Cholesterol

The paper identifies a novel bile acid biosynthesis pathway in Smith-Lemli-Opitz syndrome that bypasses cholesterol, starting from 7-dehydrocholesterol, and links intermediates to Hedgehog signaling.

Cholesterol interaction sites on the transmembrane domain of the hedgehog signal transducer and Class F G protein-coupled receptor Smoothened

The paper identifies cholesterol interaction sites on the transmembrane domain of the GPCR Smoothened using molecular dynamics simulations and free energy calculations.

Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling

The paper develops a method to rapidly deplete tubulin glutamylation in primary cilia, revealing its role in intraflagellar transport and Hedgehog signaling without affecting other PTMs.