H. Kobayashi

Phosphodiesterase-5 inhibition inhibits epithelial ATP release and restores detrusor contractility in rats with type 2 diabetes via an increase in bladder blood flow

This study shows that PDE-5 inhibition improves bladder function in T2DM rats by increasing blood flow, reducing ATP release, and modulating inflammatory and growth factor markers.

Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repair

This study shows NSD2 has dose-dependent effects on DSB repair pathways, repressing non-c-NHEJ and promoting c-NHEJ while not affecting HR or total NHEJ efficiency.

Induction of plant disease resistance by mixed oligosaccharide elicitors prepared from plant cell wall and crustacean shells

This study compares the elicitor activities of different oligosaccharides from plant cell walls and crustacean shells, showing that a mixture enhances disease resistance in tomato without inhibiting growth.

Combination therapy with oral antiviral and anti-inflammatory drugs improves the efficacy of delayed treatment in severe COVID-19

This study shows that combining an oral antiviral with a corticosteroid improves treatment outcomes in delayed SARS-CoV-2 infection in hamsters compared to monotherapy.

Colorectal cancer detection and treatment with engineered probiotics

This paper demonstrates the use of engineered probiotics to detect and treat colorectal cancer by monitoring their colonization and delivering therapeutic molecules.

Loss of Grem1-articular cartilage progenitor cells causes osteoarthritis.

This study identifies Grem1-articular cartilage progenitor cells as critical for maintaining joint health, and their loss leads to osteoarthritis, with potential therapeutic targeting of FGFR3.

Context-Dependent Modification of PFKFB3 in Hematopoietic Stem Cells Promotes Anaerobic Glycolysis and Ensures Stress Hematopoiesis

This study identifies PFKFB3 as a key regulator of glycolytic metabolism in HSCs under stress, showing its context-dependent activation promotes ATP production and hematopoiesis.

Comprehensive Analysis of Peptide-Coding Genes Identifies an LRR-only Microprotein That Regulates Reproductive Organs in Marchantia polymorpha

This study identifies an LRR-only microprotein, MpSGF10B, that regulates reproductive organ development in Marchantia polymorpha by affecting antheridiophore formation.

The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependent

This study investigates how caloric restriction affects adipose tissue and metabolic health, finding that its benefits are sex- and age-dependent in mice and humans.

Oral administration of S-217622, a SARS-CoV-2 main protease inhibitor, decreases viral load and accelerates recovery from clinical aspects of COVID-19

This study shows that oral S-217622 reduces SARS-CoV-2 viral load and improves recovery in hamsters, with activity against multiple variants.

Eliminating fibroblast activation protein-α expressing cells by photoimmunotheranostics

This study evaluates FAP-IR700 photoimmunotherapy for eliminating FAP-expressing cells in cancer models, showing effective cell killing and tumor growth inhibition.

Malaria parasite evades mosquito immunity by glutaminyl cyclase mediated protein modification

This study shows that Plasmodium sporozoites use glutaminyl cyclase to modify surface proteins, evading mosquito immune detection and melanization.

OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization

The paper maps human protein localization and interactions using genome engineering, imaging, and data science, revealing functional communities and RNA-binding protein subgroups.

Self-Supervised Deep-Learning Encodes High-Resolution Features of Protein Subcellular Localization

The paper introduces cytoself, a self-supervised deep-learning method for protein localization profiling, achieving higher clustering accuracy than existing approaches.

A flexible target-specific anti-infection therapeutic platform that can be applied to different microbial species

This paper introduces a photoimmuno-antimicrobial strategy (PIAS) that effectively targets and eliminates various pathogens, including drug-resistant strains, without harming host microflora.

Effector membrane translocation biosensors reveal G protein and β-arrestin coupling profiles of 100 therapeutically relevant GPCRs

The paper develops EMTA biosensors to profile G protein and β-arrestin coupling of 100 GPCRs, revealing diverse signaling profiles and drug-target interactions.

Discovery of Plasmodium falciparum SR12 as a GOLD-Domain seven transmembrane protein regulating GPCR trafficking in mammalian cells

The paper identifies Plasmodium falciparum SR12 as a GOST protein that promotes GPCR trafficking in mammalian cells through structural analysis and functional assays.

Infrared absorptions as indicators for Pseudomonas aeruginosa and Acinetobacter baumannii

This study uses infrared absorptions of volatile metabolites to distinguish Pseudomonas aeruginosa and Acinetobacter baumannii from other bacteria and room air.

High-Throughput Functional Evaluation of BRCA2 Variants of Unknown Significance

The paper develops a high-throughput method to evaluate BRCA2 variants of unknown significance, classifying 186 variants into neutral, intermediate, or deleterious categories with high sensitivity and specificity.

Classification of platelet aggregates by agonist type

This paper introduces a method to classify platelet aggregates by agonist type using convolutional neural networks trained on high-throughput imaging data.

Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo

This study maintains engraftable quiescent HSCs in culture for 1 month using low cytokines, hypoxia, and high fatty acids.

Electron microscopic analysis of the exo-skeleton of hadal zone amphipod Hirondellea gigas

This study examines the exoskeleton of H. gigas, revealing aluminum's role in protecting against high-pressure environments through a gel-like structure formed from aluminum hydroxide.