N. Grigorieff

Amortized template-matching of molecular conformations from cryo-electron microscopy images using simulation-based inference

This paper introduces cryoSBI, a method using simulation-based inference to infer biomolecular conformations from cryo-EM images, achieving fast and accurate results.

CTFFIND5 provides improved insight into quality, tilt and thickness of TEM samples

CTFFIND5 improves CTF estimation by incorporating sample thickness and tilt, enhancing cryoEM image quality assessment.

Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias

This paper improves 2D template matching for cryo-EM by reducing template bias and enabling high-resolution structure determination without relying on the template.

Quantification of gallium cryo-FIB milling damage in biological lamella

This paper quantifies gallium cryo-FIB milling damage in biological lamellae, showing damage extends 60 nm from each surface, limiting structural biology information recovery.

From primordial clocks to circadian oscillators

This study investigates the primordial circadian clock in Rhodobacter sphaeroides using structural and kinetic analyses to elucidate its mechanism and evolutionary origins.

Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for Label-Free Detection of Molecules across Entire Cell Sections

The paper introduces DeCo-LACE, a cryo-EM method for whole-cell section imaging, and uses 2DTM to map ribosome locations, enhancing visual proteomics.

In situ single particle classification reveals distinct 60S maturation intermediates in cells

The paper uses 2DTM to classify 60S ribosome maturation intermediates in yeast cells by analyzing their signal-to-noise ratios in cryo-EM micrographs.

Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM

This paper introduces a GPU-accelerated 2D template matching method in cisTEM for identifying macromolecular assemblies in cells, demonstrating its utility for in situ proteomics and structure determination.

Cryo-TEM simulations of amorphous radiation-sensitive samples using multislice wave propagation.

The paper introduces a new method for simulating cryo-EM images of amorphous samples, improving accuracy by modeling spatially variable inelastic scattering and radiation damage.

mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding

The paper investigates how mRNA stem-loops impede ribosome movement by hindering A-site tRNA binding and rotation during translation elongation.

Structure and assembly of calcium homeostasis modulator proteins

The paper determines the structures of CALHM1 and CALHM2 using cryo-EM and shows their unique oligomeric assembly and lipid interactions.

High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations

The paper presents high-resolution cryo-EM structures of multiple human norovirus strains, revealing size variations and structural polymorphism.

Analysis of Local Variability and Allostery in Macromolecular Assemblies using Cryo-EM and Focused Classification

The paper develops focused classification strategies for single-particle cryo-EM to analyze structural variability and allostery, applying them to an HIV-1 TAR element RNA dataset.

Structure of the ciliary axoneme at nanometer resolution reconstructed by TYGRESS

A hybrid cryo-electron microscopy method reveals subcellular structures at unprecedented resolution.

Cryo-EM structure of an amyloid fibril from systemic amyloidosis

The paper determines the cryo-EM structure of an amyloid fibril from AA amyloidosis, revealing its all-β conformation and identifying the N-terminus as critical for fibril assembly.

cisTEM: User-friendly software for single-particle image processing

The paper introduces cisTEM, a user-friendly software for single-particle image processing in electron cryo-microscopy, offering a full pipeline and efficient processing capabilities.

Mechanism of ribosome rescue by ArfA and RF2

This study reveals the structural dynamics of ribosome rescue by ArfA and RF2, showing how ArfA activates RF2 to release peptides from stalled ribosomes without a stop codon.