T. Grant

TIM3 is a context-dependent co-regulator of cytotoxic T cell function

This study shows TIM3 inhibits spheroid-suppressed CTL but not active CTL, and its function depends on the context of T cell activation and ligand expression.

Cryogenic Soft Landing Improves Structural Preservation of Protein Complexes

This paper introduces a cryogenic landing system that improves structural preservation of protein complexes for cryo-electron microscopy.

Detection of a geminate photoproduct of bovine cytochrome c oxidase by time-resolved serial femtosecond crystallography

The paper detects a geminate photoproduct of bovine cytochrome c oxidase using time-resolved serial femtosecond crystallography, revealing the ligand dissociation trajectory and protein dynamics.

Nodavirus RNA Replication Crown Architecture Reveals Proto-Crown Precursor and Viral Protein A Conformational Switching

The paper uses cryo-EM to reveal the structure of nodavirus RNA replication crowns, identifying a proto-crown precursor and conformational changes in viral protein A.

From primordial clocks to circadian oscillators

This study investigates the primordial circadian clock in Rhodobacter sphaeroides using structural and kinetic analyses to elucidate its mechanism and evolutionary origins.

Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM

This paper introduces a GPU-accelerated 2D template matching method in cisTEM for identifying macromolecular assemblies in cells, demonstrating its utility for in situ proteomics and structure determination.

Longitudinal single-cell epitope and RNA-sequencing reveals the immunological impact of type 1 interferon autoantibodies in critical COVID-19

The paper examines IFN-I autoantibodies in critical COVID-19 patients through longitudinal single-cell analysis, revealing suppressed ISG-I responses in myeloid cells.

Nurturing Diversity and Inclusion in AI in Biomedicine through a Virtual Summer Program for High School Students

The paper describes a virtual summer program for high school students aimed at increasing diversity in AI through biomedical education, showing improved knowledge and engagement in AI concepts.

Structure and assembly of calcium homeostasis modulator proteins

The paper determines the structures of CALHM1 and CALHM2 using cryo-EM and shows their unique oligomeric assembly and lipid interactions.

Evidence of Absence Treated as Absence of Evidence: The Effects of Variation in the Number and Distribution of Gaps Treated as Missing Data on the Results of Standard Maximum Likelihood Analysis

The paper evaluates how variation in gap numbers and distributions affects maximum likelihood analysis results, finding negative correlations between ML scores and gap opening costs and missing data.

High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations

The paper presents high-resolution cryo-EM structures of multiple human norovirus strains, revealing size variations and structural polymorphism.

CryoEM structures of open dimers of Gyrase A in complex with DNA illuminate mechanism of strand passage

The paper presents cryoEM structures of open gyrase A dimers with DNA, revealing intermediate states of the catalytic cycle and DNA interactions.

cisTEM: User-friendly software for single-particle image processing

The paper introduces cisTEM, a user-friendly software for single-particle image processing in electron cryo-microscopy, offering a full pipeline and efficient processing capabilities.

Enzyme Intermediates Captured "on-the-fly" by Mix-and-Inject Serial Crystallography

This paper demonstrates a method to capture enzyme reactions in real-time using mix-and-inject serial crystallography, revealing antibiotic cleavage and kinetic transitions in Mycobacterium tuberculosis β-lactamase.

Mechanism of ribosome rescue by ArfA and RF2

This study reveals the structural dynamics of ribosome rescue by ArfA and RF2, showing how ArfA activates RF2 to release peptides from stalled ribosomes without a stop codon.