S. Ghaemmaghami

Formylation facilitates the reduction of oxidized initiator methionines

This study shows that formylation of initiator methionines enhances their enzymatic reduction and mitigates oxidation-related misprocessing.

Proteome birthdating reveals age-selectivity of protein ubiquitination

This study introduces proteome birthdating to measure protein age and finds that ubiquitinated proteins are predominantly newly synthesized, with distinct age distributions across cellular pathways.

Methionine Alkylation as an Approach to Quantify Methionine Oxidation Using Mass Spectrometry

This paper introduces MObBa, a mass spectrometry method to quantify methionine oxidation by alkylating unoxidized methionines, enabling accurate measurement of oxidation levels in peptides and proteomes.

Long lifetime and selective accumulation of the A-type lamins accounts for the tissue specificity of Hutchinson-Gilford progeria syndrome

This study shows that the long lifetime and selective accumulation of A-type lamins in specific tissues explain the tissue specificity of Hutchinson-Gilford progeria syndrome.

Folding stabilities of ribosome-bound nascent polypeptides probed by mass spectrometry

This study uses mass spectrometry to measure how ribosome binding affects the folding stability of nascent polypeptides, finding that ribosome-induced stability changes depend on localized charge distributions.

Accurate proteome-wide measurement of methionine oxidation in aging mouse brains

This study introduces a method to measure methionine oxidation in mouse brains and finds that oxidation is consistent across individuals but not significantly altered with age.

Protein folding stabilities are a major determinant of oxidation rates for buried methionine residues

The paper shows that oxidation rates of buried methionines correlate with protein folding stability, using E. coli and T. thermophilus proteomes to demonstrate this relationship.

MicroRNA-574 Regulates FAM210A Expression and Influences Pathological Cardiac Remodeling

This study identifies miR-574 as a regulator of FAM210A, which influences mitochondrial protein expression and cardiac remodeling in heart failure.

Redox-mediated regulation of a labile, evolutionarily conserved cross-β structure formed by the TDP43 low complexity domain

This study shows that redox regulation controls TDP43 low complexity domain aggregation into cross-β polymers, with implications for neurodegenerative diseases.

Quantitative analysis of in vivo methionine oxidation of the human proteome

This study develops a method to quantify methionine oxidation in the human proteome by isotopic labeling and validates it using artificial proteomes, identifying novel in vivo oxidation sites.

Global analysis of protein degradation in prion infected cells

This study quantifies how prion infection affects protein degradation rates in N2a cells, finding increased degradation kinetics for most proteins and reduced prion protein degradation.

Global analysis of methionine oxidation provides a census of folding stabilities for the human proteome

The paper quantifies protein folding stabilities in human cell extracts using methionine oxidation rates, identifying stable ontological subsets and showing stability impacts oxidation and TMAO effects.

Potential Mechanisms Linking SIRT Activity And Hypoxic 2-Hydroxyglutarate Generation: No Role For Direct Enzyme (De)acetylation

This study investigates whether sirtuin-mediated acetylation regulates 2-HG production by metabolic enzymes, finding no direct link between enzyme acetylation and 2-HG generation.