David Gatfield

Low- and high-dose anisomycin confine ribosome collisions to a dose-shifted zone just past the start codon, whereas 30 min after UV, collisions appear more dispersed and favor highly translated mRNAs.

The paper investigates the relationship between allele-specific expression and translational efficiency in mouse embryos and stem cells, finding lower translational efficiency associated with allelic imbalance.

The paper investigates how SMG5 licenses SMG6 endonuclease activity in NMD by forming a composite active site with SMG6 PIN domain.

This study identifies BUD23 as a regulator of energy homeostasis by controlling translation of mitochondrial and lipogenic mRNAs, linking ribosomal modification to metabolic health.

The paper shows that Pus7 mutations reduce tRNA-Asp levels, activating the integrated stress response and glycolytic reprogramming, which contributes to aggressive behavior.

This study shows that SMG6-dependent NMD prevents dsRNA accumulation and immunogenicity in HCC by maintaining dsRNA homeostasis and suppressing MHC-I-presented peptides.

The paper develops an in vitro re-initiation assay and uses ribosome profiling to show that MCTS2 and eIF2D have distinct roles in uORF-dependent translation.

The paper investigates diurnal regulation of IRE-containing mRNAs via IRP1 and IRP2 in mouse liver, showing that feeding rhythms drive rhythmicity even without a functional circadian clock.

This study uses a Smg6 mouse model to show that NMD pathway downregulation affects circadian period length and daily CRY2 mRNA accumulation.

The paper identifies that only 6% of expressed genes are functionally regulated by miRNAs in mESCs using integrative analysis of transcriptome data and miRNA interaction predictions.

This study presents the cryo-EM structure of the mammalian ribosome during SARS-CoV-2 RNA translation, revealing the pseudoknot structure that facilitates ribosomal frameshifting and identifies a compound that inhibits viral replication.

The paper identifies DENR-dependent translation reinitiation events and links uORF usage to circadian clock regulation by analyzing ribosome profiling data in DENR-deficient cells.

The paper investigates how translation efficiency contributes to tissue-specific rhythmic and constitutive gene expression in kidney and liver, revealing translational control mechanisms and compensation effects.