Shyam Sundar Krishnakumar

The paper compares mammalian and C. elegans orthologs of Synaptotagmin-1 and Complexin in a single-vesicle assay to understand their roles in synaptic vesicle clamping and neurotransmitter release.

The paper identifies a minimal set of presynaptic proteins that regulate different modes of Ca2+-triggered vesicle fusion and short-term plasticity.

The paper reconstitutes core synaptic machinery to reveal DAG's role in vesicle priming and Ca2+-triggered release, showing DAG accelerates SNAREpin assembly and affects release dynamics.

The paper combines mathematical modeling and experiments to show Synaptotagmin rings act as Ca2+-sensitive fusion clamps and docking sensors in synaptic vesicle release.

The paper shows Munc13-1 directly binds SNAP25 to initiate SNARE complex assembly, using FRET and optical tweezer techniques.

This study shows that Syt1 oligomerisation is essential for clamping spontaneous and asynchronous neurotransmitter release, revealing a mechanism for regulating release modes.

This study investigates the independent yet synergistic roles of Synaptotagmin-1 and Complexin in calcium-regulated neuronal exocytosis using a reconstituted single-vesicle fusion assay.

This paper determines the structure of the Syt1-SNARE complex on lipid membranes using cryo-electron microscopy to elucidate how Syt1 clamps and activates SNARE-mediated fusion through Ca2+ and Mg2+ dependent interactions.

This study investigates how mutations in Membrin/GOSR2 affect dendritic growth and synaptic integrity in Drosophila models, revealing their role in neurological disorders.