The paper investigates the role of RNase H types 1 and 2 in removing ribonucleotides from the genomes of two archaeal species using combined experimental and sequencing approaches.

Nucleic Acids Research · Nov 13, 2025Peer-reviewedGenomics

Genome wide analyses reveal the role of mutator phenotypes in the emergence of drug resistance in Mycobacterium tuberculosis

Rima Zein-Eddine, Adrien Le Meur, Stéphane Skouloubris, Lars Jelsbak, Guislaine Refrégier, Hannu Myllykallio

The paper identifies 18 novel SNPs in DNA repair genes linked to drug resistance in Mtb using genome-wide analysis of 53,589 whole-genomes.

npj Antimicrobials and Resistance · Apr 29, 2025Peer-reviewedEvolutionary Biology

Enabling large-scale genome editing by reducing DNA nicking

Cory J. Smith, Oscar Castanon, Khaled Said, Verena Volf, Parastoo Khoshakhlagh, Amanda Hornick, Raphaël Ferreira, Chunting Wu, Marc Güell, Shilpa Garg, Alex H. M. Ng, Hannu Myllykallio, George McDonald Church

This paper develops dead-Cas9 base editors that enable large-scale genome editing in human cells by reducing DNA nicking, allowing editing of over 10,000 loci.

Nucleic Acids Research · Apr 1, 2020Peer-reviewedSynthetic Biology

CRISPR-mediated biocontainment

Oscar Castanon, Cory J. Smith, Parastoo Khoshakhlagh, Raphaël Ferreira, Marc Güell, Khaled Said, Ramazan Yildiz, Matthew Dysart, Stan Wang, David B. Thompson, Hannu Myllykallio, George McDonald Church

The paper develops CRISPR-based biocontainment circuits using transposable elements to induce cell death and create kill-switches for controlling cell lines.

bioRxiv · Feb 3, 2020 · PreprintSynthetic Biology