Aartjan J.W. te Velthuis

Defective Viral Genomes Reveal History-dependent Self-regulation of Type I Interferon Dynamics

The paper develops a Bayesian model to show that defective viral genomes modulate type I interferon dynamics by both inducing and suppressing IFN through cumulative exposure, explaining how pandemic viruses evade innate immunity.

Intersegmental transfers drive target search in an RNA-targeting CRISPR system

This study shows that Cas13 uses intersegmental transfers to efficiently search for RNA targets, differing from DNA-binding proteins.

Spatio-temporal modelling of in vitro viral infection: the impact of defective interfering particles on type I interferon response

The paper develops a spatially explicit model of in vitro viral infection with DIPs, showing how they affect IFN response and plaque morphology.

Febrile temperature activates the innate immune response by promoting aberrant influenza A virus RNA synthesis

This study shows that fever during influenza A virus infection increases antiviral signaling by promoting aberrant RNA synthesis and innate immune activation.

Amplification-free Detection of Zoonotic Viruses Using Cas13 and Multiple CRISPR RNAs

This study develops an amplification-free CRISPR-Cas13 assay for detecting zoonotic viruses and finds that multiple crRNAs improve sensitivity for some viruses but not others.

RNA folding drives species-dependent host mRNA partitioning in Ebola virus condensates

The paper investigates how RNA folding influences host mRNA partitioning into Ebola virus condensates under different conditions and identifies implications for viral-host co-evolution.

A two-step mechanism for RIG-I activation by influenza virus mini viral RNAs

This study identifies a two-step mechanism by which influenza virus mini viral RNAs (mvRNAs) activate RIG-I through non-canonical transcription and RNA structure-dependent binding.

Zinc ions inactivate influenza virus hemagglutinin and prevent receptor binding

This study shows zinc ions inhibit influenza virus receptor binding and prevent hemagglutinin function, with no resistance observed after serial passaging.

Efficient genome replication in influenza A virus requires NS2 and sequence beyond the canonical promoter

The paper identifies NS2-dependent sequence requirements beyond canonical promoters for efficient influenza A virus genome replication, explaining divergent transcriptional dynamics across viral segments.

Deep mutational scanning of influenza A virus NEP reveals pleiotropic mutations in its N-terminal domain

This study uses deep mutational scanning to analyze the functional impact of NEP mutations in influenza A virus, revealing pleiotropic effects on viral replication and host adaptation.

Influenza A virus transcription generates capped cRNAs that activate RIG-I

This study identifies a novel IAV RNA species, capped complementary RNAs (ccRNAs), which hybridize with viral RNA to activate RIG-I, revealing an alternative mechanism for innate immune activation during IAV infection.

RNA structure modulates Cas13 activity and enables mismatch detection

This study investigates how RNA structure affects Cas13 activity, revealing two mechanisms by which secondary structure modulates Cas13 and improving RNA detection through strand displacement.