Clifford P. Brangwynne

Deep Learning of Functional Perturbations from Condensate Morphology

This paper introduces Deep-Phase, a deep learning framework that classifies and quantifies drug-induced changes in condensate morphology from microscopy images, revealing insights into nucleolar structure and function.

Enhanced GPP synthesis by Erg20p-peptide fusions and biomolecular condensates boosts monoterpene production in yeast

The paper enhances monoterpene production in yeast by using Erg20p-peptide fusions and biomolecular condensates to increase GPP synthesis over FPP synthesis.

Ribosome biogenesis bottlenecks reveal vulnerabilities in cancer

This study identifies late-stage processing bottlenecks in ribosome biogenesis caused by oncogene activation, which impair ribosome production and limit tumor growth.

Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions

This study identifies the kinase KEY1 as a regulator of pyrenoid condensate size and dynamics in Chlamydomonas reinhardtii, showing it disrupts phase separation interactions to control condensate dissolution during the cell cycle.

Ribosome Molecular Aging Shapes Translation Dynamics

The paper shows that molecularly aged ribosomes alter translational dynamics by impairing elongation and increasing ribosome collisions during organismal aging.

Metabolism of epigenetic ribonucleosides leads to nucleolar stress and cytotoxicity

This study investigates the metabolism of epigenetic ribonucleosides in human cells, revealing cytotoxic effects and mechanisms of RNA misincorporation and nucleolar stress.

RNA folding drives species-dependent host mRNA partitioning in Ebola virus condensates

The paper investigates how RNA folding influences host mRNA partitioning into Ebola virus condensates under different conditions and identifies implications for viral-host co-evolution.

Mapping and engineering RNA-controlled architecture of the multiphase nucleolus

The paper maps rRNA processing dynamics in the nucleolus and shows how rRNA processing affects nucleolar phase organization and structure.

Condensate-driven chromatin organization via elastocapillary interactions

This study develops a mesoscopic model showing how condensates interact with chromatin through elastocapillary forces, influencing chromatin organization and function.

Genome-wide mapping of mesoscale neuronal RNA organization and condensation

The paper introduces RNA-SPRITE to map RNA-RNA spatial proximity genome-wide, revealing conserved mRNA organization and colocalization driven by shared motifs and functional relationships.

Squid primary cell culture as a model system and experimental tool

This paper describes a protocol for isolating and culturing squid primary cells from various tissues and life stages to study squid biology and physiology.

Interplay of condensation and chromatin binding underlies BRD4 targeting

This study investigates how BRD4 chromatin binding modulates its condensate formation, showing that chromatin interactions influence both kinetic and thermodynamic properties of BRD4 condensation.

Live imaging of Alu elements reveals non-uniform euchromatin dynamics coupled to transcription

The paper uses CRISPR to live image Alu elements in euchromatin, revealing non-uniform dynamics linked to transcriptional activity.

A Modular Design for Synthetic Membraneless Organelles Enables Compositional and Functional Control

This paper introduces a modular framework for synthetic membraneless organelles that enables compositional and functional control through decoupled cluster formation and protein recruitment.

An FGF Timer for Zygotic Genome Activation

The paper studies the timing of zygotic genome activation in Ciona intestinalis using FGF signaling and finds that two Prdm1 homologs are the earliest genes induced, with a sharp transition in FGF responsiveness between 8- and 16-cell stages.

Regulation of gene expression by repression condensates during development

The paper investigates the role of liquid-liquid phase separation in transcriptional repression by studying Hes/Gro complexes in Ciona embryos and their behavior during cell cycles.

Protein phase separation provides long-term memory of transient spatial stimuli

The paper shows protein droplets retain spatial memory of transient stimuli, demonstrating this through computational models, optogenetic systems, and membrane signaling.

Microfluidic Immobilization And Subcellular Imaging Of Developing Caenorhabditis elegans

This paper introduces a microfluidic device to immobilize and image subcellular structures in C. elegans over time, enabling FRAP measurements without chemical anesthetics.