Robert B. Darnell

This study uses single-cell multi-omics to identify OTP and NOVA1 as key regulators of leptin action in hypothalamic feeding circuits, revealing cell-type-specific transcriptional and regulatory changes.

This study identifies eIF4G2-dependent local translation as a mechanism linking synaptic activity to mitochondrial adaptation in dendrites.

The paper identifies four synovial transcriptional clusters in knee OA linked to cartilage degeneration severity through bulk and single-cell RNA-seq analyses.

The paper investigates how aging-dependent dysregulation of EXOSC2 affects cellular traits and finds that PPP2R5E, a PP2A phosphatase subunit, is a key target of EXOSC2, with this regulation maintained in stem cells and cancer.

The paper identifies ELAVL3 as a key RNA-binding protein regulating alternative splicing of synaptic signaling proteins in D1 and D2 striatal medium spiny neurons.

This study uses Opto-CLIP to examine how FMRP regulates mRNAs in CA1 neurons after activation, showing distinct dynamics in cell body and synapse.

This study identifies miR-218 as critical for hippocampal network stability by regulating interneuron function during early development.

This study shows that Nova proteins, through activity-dependent alternative splicing, are essential for the maturation and connectivity of somatostatin interneurons.

The paper identifies compartment-specific FMRP targets in CA1 pyramidal neurons and shows how FMRP regulates different mRNA functions in cell bodies and dendrites.

The paper identifies NOVA2 as a key regulator of circRNA biogenesis in the developing mouse brain, showing its role in enhancing circRNA levels through specific binding sites.

The paper identifies RBP target site dysregulation as a major contributor to psychiatric disorder risk, revealing new insights into disease heritability and providing a resource for further research.

The paper identifies three novel complex structural variation patterns in cancer genomes using genome graph analysis across 2,833 tumor WGS.

The paper develops a mouse model to identify FMRP mRNA targets in specific neurons, revealing that FMRP binds to a ranked subset of long genes related to Fragile X phenotypes.

The paper uses AGO CLIP to identify miRNA-target interactions in the brain after ischemic stroke, revealing miR-29 family members lose target regulation, leading to rapid proteomic changes in glutamate homeostasis.

The paper uses deep learning to analyze whole-genome data from ASD families, showing noncoding mutations significantly impact transcriptional and post-transcriptional regulation and contribute to autism.

This study examines m6A dynamics during early vertebrate embryogenesis, finding increased m6A in polyadenylated mRNA and its association with translational efficiency and RNA degradation.

The paper develops a method to study cell-type specific RNA regulation in motoneurons and finds NOVA regulates cytoskeleton interactions by promoting a specific isoform of Septin 8.

This study identifies ZFP36 RNA-binding proteins as regulators of T-cell activation and anti-viral immunity, showing they restrain T-cell expansion and effector functions.

The paper identifies differences in alternative polyadenylation (APA) between mouse cerebellar neuron types and shows how APA affects gene regulation and function.

This study identifies pharmacogenetic variants associated with albuterol bronchodilator response in minority children with asthma using whole genome sequencing.

The paper develops a low-cost microfluidic instrument for scRNA-seq and applies it to profile synovial tissue from a rheumatoid arthritis patient, identifying 16 transcriptomic clusters and fibroblast subpopulations.

The paper uses NOVA2 cTag-CLIP to study RNA regulation in specific neurons, revealing cell-type differences in RBP actions and their impact on RNA diversity and function.

The paper identifies de novo intronic indels in autism target genes from simplex families and finds they contribute to at least 10% of simplex autism.