Miriam Stoeber

An engineered nanobody inhibitor for molecular-to-circuit control of opioid receptor function

This paper develops an engineered nanobody inhibitor to control opioid receptor function with subcellular precision and demonstrates its effectiveness in vivo.

Visualizing the Dominant GPCR Coupling of Pathogenic Gαo Mutants in GNAO1-Related Disorders

This study uses a split-YFP BiFC assay to show that severe GNAO1 mutations impair GPCR disengagement, while milder mutants show near-normal receptor internalization.

CRISPR screen identifies CNIH1 as a selective driver of GPCR export

The paper identifies CNIH1 as a key factor for GPCR export using a CRISPR screen and cargo-release assay, showing its role in transporting DOR to the plasma membrane.

Structural Basis of μ-Opioid Receptor-Targeting by a Nanobody Antagonist

This paper describes a nanobody antagonist of the μ-opioid receptor, revealing its structural basis and designing peptide analogues with antagonistic activity.

Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide release

This paper develops a genetically encoded sensor, NOPLight, to detect endogenous N/OFQ release with high spatial and temporal resolution in vitro and in vivo.

Probing PAC1 receptor activation across species with an engineered sensor

The paper introduces a novel genetically-encoded sensor for PAC1 receptor activation, demonstrating its utility in vitro and in vivo across species.

A genetically encoded biosensor reveals location bias of opioid drug action

This study develops a biosensor to map opioid receptor activation in neurons, showing drugs activate receptors in the Golgi and dendrites, while peptides activate them in the plasma membrane and endosomes.