Michael Lidschreiber

Structure of human cytoplasmic Pol II complex explains global transcription repression by Gdown1

The paper determines the cryo-EM structure of cytoplasmic Pol II and shows Gdown1 inhibits its transcriptional activity by blocking essential factor binding.

Mechanism of Co-Transcriptional Cap-Snatching by Influenza Polymerase

This study reveals the molecular mechanisms of cap-snatching by influenza polymerase using cryo-EM structures of FluPol bound to Pol II and DSIF.

CTCF depletion decouples enhancer-mediated gene activation from chromatin hub formation during cellular differentiation

The paper shows that CTCF depletion disrupts chromatin hub formation but not enhancer-promoter interactions, which still drive gene expression during differentiation.

The Mediator complex regulates enhancer-promoter interactions

This study shows that the Mediator complex is essential for enhancer-promoter interactions, as its depletion reduces interaction frequencies and gene expression, while increasing CTCF-binding site interactions and altering Cohesin distribution.

Oct4 primarily controls enhancer activity rather than accessibility

This study shows Oct4 primarily activates transcription of pluripotency enhancers rather than controlling their chromatin accessibility.

Sequence determinants of human gene regulatory elements

The paper identifies sequence determinants of human gene regulatory elements by analyzing transcriptional activity of long DNA sequences in three cell types, revealing distinct enhancer types and TF subtypes based on motif content and activity patterns.

Newly synthesized RNA Sequencing Characterizes Transcription Dynamics in Three Pluripotent States

This study uses TT-seq to analyze RNA synthesis dynamics in three mouse pluripotent states, finding reduced RNA synthesis and elongation velocity in 2i and mTORi states.

NASC-seq monitors RNA synthesis in single cells

NASC-seq monitors newly synthesized RNA in single cells, revealing dynamic gene expression during T-cell activation.