Chong Tang

Exploring DNA movement through the application of droplet based high efficient chromatin conformation capture (DropHiChew) and loop velocity

This paper introduces DropHiChew and loop velocity to study DNA movement dynamics, enabling accurate trajectory estimation with shallow sequencing.

High efficient chromatin conformation capture without pre-enrichment (HiChew) in single cells

HiChew improves chromatin conformation capture in single cells with higher efficiency, fewer false positives, and more contacts per cell compared to Dip-C.

Spatial chromatin accessibility sequencing resolves high-order spatial interactions of epigenomic markers

This study introduces SCA-seq to resolve spatial chromatin interactions at single-molecular resolution and demonstrates its application in analyzing epigenomic markers.

Improving the RNA velocity approach using long-read single cell sequencing

The paper improves RNA velocity analysis by incorporating long-read single-cell sequencing to better capture gene expression dynamics and cell fate transitions.

High coverage of single cell genomes by T7-assisted enzymatic methyl-sequencing

This study introduces TEAM-seq, a method for high-coverage 5mC sequencing in single cells, achieving 70% coverage at 100pg input DNA.

Long-range single-molecule mapping of chromatin modification in eukaryotes

The paper introduces a method called BIND&MODIFY to map histone modifications at the single-molecule level using long-read sequencing, revealing long-range chromatin states and heterogeneity.

Sequencing of methylase-accessible regions in integral circular extrachromosomal DNA reveals differences in chromatin structure.

The paper introduces CCDA-seq to study ecDNA chromatin structure, revealing distinct nucleosome and regulator binding patterns.

AASRA: An Anchor Alignment-Based Small RNA Annotation Pipeline

AASRA is a small RNA annotation pipeline that improves accuracy and speed by handling sncRNA variants and distinguishing mature from precursor miRNAs.

miRNA-dependent poly(A) length control in uncoupling transcription and translation of haploid male germ cells

The paper identifies miRNA-dependent poly(A) length regulation as a mechanism for uncoupling transcription and translation in spermatids.

Maternal transmission of a paramutant phenotype requires intact DNMT2 functions in the male germline

The paper investigates the role of DNMT2 in maternal transmission of a paramutant phenotype through the male germline.

HIT-scISOseq: High-throughput and High-accuracy Single-cell Full-length Isoform Sequencing for Corneal Epithelium

HIT-scISOseq improves single-cell isoform sequencing throughput and accuracy, revealing novel transcriptomic features in corneal epithelium injury.

A new massively parallel nanoball sequencing platform for whole exome research

This study evaluates the BGISEQ-500 platform for whole exome sequencing, finding it comparable to Hiseq4000 in SNP detection and slightly better in indel detection.

Template switching causes artificial junction formation and false identification of circular RNAs

The paper identifies artificial junction sequences formed by reverse transcriptases, leading to false circular RNA identification, and proposes methods to distinguish true circular RNAs.