Orna Dahan

A massively parallel reporter screen defines the syntax of SECReTE, a secretion-enhancing cis regulatory targeting element, in mammalian cells

The paper identifies the syntax of SECReTE, a secretion-enhancing cis regulatory element, through a parallel reporter screen in mammalian cells, revealing key sequence patterns and their impact on mRNA localization and protein secretion.

Evolution of a genome-architecture-encoded gene regulation system in trypanosomatids

This study investigates the evolution of gene regulation in trypanosomatids, showing a shift from gene-specific regulation to architecture-encoded regulation through polycistronic transcription units.

Essentiality and dynamic expression of the human tRNA pool during viral infection

This study examines how HCMV and SARS-CoV-2 affect the human tRNA pool and finds HCMV alters tRNA expression and modifications, while SARS-CoV-2 does not, with implications for viral codon usage and host-virus interactions.

The evolutionary origin of host association and polycistronic transcription in trypanosomatids

This study investigates the evolutionary origins of host association and polycistronic transcription in trypanosomatids using comparative genomics across 47 genomes.

Encoded and non-genetic alternative protein variants expand human functional proteome

The paper identifies and characterizes non-genetic and genetic protein variants in human proteomes, showing they contribute to functional diversity.

Quantitative genetics of natural S. cerevisiae strains upon sexual mating reveals heritable determinants of cellular fitness

The paper analyzes hybrid fitness in S. cerevisiae strains under different carbon sources, finding correlations with parental fitness and genetic distance.

Dynamic changes in tRNA modifications and abundance during T-cell activation

The paper examines dynamic changes in tRNA modifications and abundance during T-cell activation, finding reduced wybutosine and ms2t6A modifications that may increase ribosomal frameshifting and facilitate HIV infection.

Sequence determinants and evolution of constitutive and alternative splicing in yeast species

The paper investigates how intronic sequence features influence splicing efficiency and mRNA expression in yeast, revealing evolutionary adaptations and mechanisms of alternative splicing.

Manipulation of the human tRNA pool reveals distinct tRNA sets that act in cellular proliferation or cell cycle arrest

The paper identifies tRNA subsets essential for cellular proliferation or arrest by CRISPR-based editing and functional analysis in human cell lines.

Systematic detection of amino acid substitutions in proteome reveals a mechanistic basis of ribosome errors

The paper systematically detects amino acid substitutions in proteomes using mass spectrometry data and reveals that translation errors are primarily caused by codon-anticodon mispairing, with patterns influenced by ribosome velocity and evolutionary constraints.