Andrew N. Holding

Aberrant splicing of MBD1 reshapes the epigenome to drive convergent myeloerythroid defects in MDS

This study identifies MBD1-L, an aberrantly spliced transcript in MDS, which drives epigenetic changes leading to myeloerythroid defects, and shows ASO-based targeting can restore erythroid differentiation.

HIF-1 activated by PIM1 assembles a pathological transcription complex and regulon that drives JAK2V617F MPN disease

This study identifies that HIF-1 activation by PIM1 in JAK2V617F-MPNs drives non-canonical transcription complex formation and disease progression.

An ERα-Dependent Hypoxia Response Defines EMT-Adjacent Tumour Regions and Suppresses the Pro-survival Effects of Amiloride in Estrogen Receptor-Positive Breast Cancer

The paper identifies an ERα-dependent hypoxia response in ER+ breast cancer that influences gene expression and sensitivity to amiloride, linking hypoxia and ER signaling.

DIANNeR: label-free RIME resolves lineage-restricted Glucocorticoid Receptor interactomes in normal human tissues

DIANNeR improves detection of GR interactomes in normal tissues by integrating DIA, ion mobility, and library-free analysis, revealing context-specific networks and previously undetected interactions.

Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell lines

This study identifies RPLP1 and RPL27 as robust reference genes for RT-qPCR analysis of gene expression in hypoxic breast cancer cell lines.

Selection of pre-leukemic hematopoietic stem cells driven by distinct extracellular matrix molecules

The paper characterizes Tet2-mutant HSCs using multi-omic data and identifies ECM molecules as regulators of self-renewal in pre-leukemic HSCs.

Novel Quantitative ChIP-seq Methods Measure Absolute Fold-Change in ER Binding Upon Fulvestrant Treatment

The paper introduces a novel normalization strategy for ChIP-seq to measure absolute fold-change in ER binding, validated using fulvestrant treatment and other experimental conditions.