Robert J. Klose

Nuclear-BRITE reduces background noise in MS2-based live-cell imaging of nuclear RNAs without affecting their abundance or function.

The paper identifies mTOR's role in regulating developmental gene promoters in pluripotent mouse embryonic stem cells through interaction with PRC1 and RNA Polymerase II pausing.

The paper identifies a new SET1B complex and shows SET1/MLL complexes control transcription independently of H3K4me3 by counteracting promoter-proximal termination and supporting transcription burst size.

The paper identifies H3K27me3's dual role in regulating transposons and cell type-specific genes in choanoflagellates, suggesting ancestral chromatin mechanisms in development.

This study investigates DNA methylation dynamics during lamprey embryogenesis, revealing extensive maternal-to-paternal epigenome remodeling and its link to programmed genome rearrangement.

The paper identifies a CpG island-encoded mechanism where SET1 complexes interact with WDR82 to prevent premature transcription termination, distinguishing genic from non-genic transcription.

The paper shows that Polycomb repression during S/G2 phases restricts differentiation gene activation, allowing it to occur primarily in G1 phase in mouse ESCs.

The paper investigates the role of CDK-Mediator in gene regulation, finding it essential for Polycomb-dependent chromosomal interactions and gene priming during differentiation through distinct mechanisms.

This study identifies PRC1's role in Polycomb-mediated gene repression by showing it independently counteracts RNA polymerase II binding and controls transcriptional burst frequency.

This study uses live-cell single particle tracking to show PRC1 has low chromatin occupancy and dynamic interactions, suggesting chromatin modification, not complex occupancy, drives gene repression.

The paper shows that BAP1 limits H2AK119ub1 levels, which affects gene expression and Polycomb complex function, revealing a general mechanism for controlling transcriptional potential.

This study investigates the role of PRC1 catalytic activity in Polycomb system function, showing it is essential for chromatin domain formation and gene repression.

This study shows KDM2 proteins repress transcription from CpG island promoters without relying on their histone demethylase activity.

The paper investigates how DNA methylation and histone acetylation independently affect transcription factor binding and gene expression in mouse embryonic stem cells.

The paper shows cohesin disrupts polycomb-dependent chromosomal interactions to regulate gene repression in embryonic stem cells.

The paper investigates the role of PcG proteins in chromatin accessibility and nucleosome organization in mouse embryonic stem cells, finding that PcG proteins do not significantly affect chromatin accessibility but influence nucleosome occupancy and spacing.

The paper identifies FBXL19 as a CpG island-binding protein that recruits CDK-Mediator to developmental gene promoters in mouse ES cells, priming them for activation during lineage commitment.