Edward Ward

MitoDecode: quantitative phenotypic decoding of mitochondrial stress states from fluorescence images

MitoDecode assesses mitochondrial stress types and severity from fluorescence images without segmentation, showing adaptability across cell types and stress conditions.

Antarctic fish cell cultures show adaptation of organelle morphology and dynamics to extreme cold

The paper studies subcellular organization and dynamics in Antarctic fish cell cultures, finding conserved organelle structures but differences in lysosomal size and mitochondrial morphology that may relate to cold adaptation.

α-Synuclein Facilitates Spontaneous Dopamine Release in a Calcium- and Phosphorylation-Dependent Manner

This study shows α-synuclein modulates spontaneous dopamine release via calcium and phosphorylation-dependent mechanisms, independent of full-fusion recycling.

SIMple: A fibre-based platform for accessible structured illumination microscopy

The paper introduces a fibre-based structured illumination microscopy (SIM) system that simplifies setup and improves resolution without specialized optics.

An Enhanced Mountain Climbing Search Algorithm to Enable Fast and Accurate Autofocusing in High Resolution Fluorescence Microscopy

This paper introduces an enhanced mountain climbing search algorithm for fast and accurate autofocus in high-resolution fluorescence microscopy, achieving up to 200-fold improvement in precision over existing methods.

Deep learning for fluorescence lifetime predictions enables high-throughput in vivo imaging

FLIMngo, a deep learning model, enables fast and accurate fluorescence lifetime predictions from low-photon FLIM data, improving throughput and reducing phototoxicity for in vivo applications.

Brain cholesterol metabolites cause significant neurodegeneration in human iPSC-derived neurons

This study shows that 24S-HC and 27-HC, brain cholesterol metabolites, cause neuronal dysfunction in human iPSC-derived neurons by disrupting calcium signaling, organelle function, and alpha-synuclein accumulation.

FLIMPA: A versatile software for Fluorescence Lifetime Imaging Microscopy Phasor Analysis

FLIMPA is an open-source software for FLIM phasor analysis that integrates data from multiple trials and provides tools for quantifying microtubule depolymerisation.

A high-resolution microscopy system for biological studies of cold-adapted species under physiological conditions

This paper develops a high-resolution microscopy method for imaging live biological samples at sub-zero temperatures to study cold-adapted species under physiological conditions.

Graphene microelectrode arrays, 4D structured illumination microscopy, and a machine learning-based spike sorting algorithm permit the analysis of ultrastructural neuronal changes during neuronal signalling in a model of Niemann-Pick disease type C

α-synuclein fibril and synaptic vesicle interactions lead to vesicle destruction and increased uptake into neurons

This study investigates how monomeric and fibrillar alpha-synuclein interact with synaptic vesicles, showing fibrils disintegrate vesicles and increase neuronal uptake, potentially contributing to Parkinson's disease pathology.

Label-free characterisation of amyloids and alpha-Synuclein polymorphs by exploiting their intrinsic fluorescence property

This paper introduces a label-free method using intrinsic fluorescence lifetime to characterize amyloids and their polymorphs, showing distinct fluorescence lifetimes for different amyloid structures and polymorphic populations.