This study shows α-synuclein modulates spontaneous dopamine release via calcium and phosphorylation-dependent mechanisms, independent of full-fusion recycling.

bioRxiv · Apr 22, 2026 · PreprintNeuroscience

A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development

Cydric Geyskens, Efstathia Kotoula, Kjen Bogaert, Elke Leysen, Joris Vandenbempt, Francisco Pestana, L. J. LeFever, Pedro Magalhães, Dan Dascenco, Joris de Wit

The paper identifies VCAM1 as an astrocytic regulator of hippocampal synapse development through cross-modal analysis combining single-cell spatial transcriptomics and protein-level profiling.

bioRxiv · Mar 18, 2026 · PreprintNeuroscience

LFQ Benchmark Dataset - Generation Beta: Assessing Modern Proteomics Instruments and Acquisition Workflows with High-Throughput LC Gradients

Bart Van Puyvelde, Robbe Devreese, Cristina Chiva, Eduard Sabidó, Sibylle Pfammatter, Christian Panse, Jeewan Babu Rijal, Charline Keller, Ihor Batruch, Patrick A. Pribil, Jean-Baptiste Vincendet, Frédéric‐Georges Fontaine, L. J. LeFever, Pedro Magalhães, Dieter L. D. Deforce, , Maarten Dhaenens

The paper generates a benchmark dataset to evaluate proteomics instruments and workflows using high-throughput LC gradients across multiple platforms and organisms.

bioRxiv · Feb 9, 2026 · PreprintBioinformatics

Intrabody-guided synapse proteomics defines pyramidal neuron input architecture and uncovers early remodeling in a mouse model of Alzheimer's disease

Dan Dascenco, Gabriele Marcassa, Anastasia Mallopoulou, Jeroen Vandensteen, L. J. LeFever, Elke Leysen, Blanca Lorente-Echeverría, Artemis Koumoundourou, Danie Daaboul, Bart Ghesquière, Pedro Magalhães, Joris de Wit

The paper uses an intrabody-guided proximity-labeling strategy to map postsynaptic proteomes in hippocampal CA1 pyramidal neurons and identifies early synaptic remodeling in an Alzheimer's disease mouse model.

bioRxiv · Nov 24, 2025 · PreprintNeuroscience

Neighbouring modifications interfere with the detection of phosphorylated alpha-synuclein at Serine 129: Revisiting the specificity of pS129 antibodies

Hilal Ahmed Lashuel, Anne‐Laure Mahul‐Mellier, Salvatore Novello, Ramanath Narayana Hegde, Yllza Jasiqi, Melek Firat Altay, Sonia Donzelli, Sean M. DeGuire, Ritwik Burai, Pedro Magalhães, Anass Chiki, Jonathan Ricci, Manel L. N. Boussouf, Ahmed Sadek, Erik Stoops, , Nicolas Guex

The paper shows that neighboring post-translational modifications affect the detection of phosphorylated alpha-synuclein at Serine 129 by existing antibodies.

bioRxiv · Mar 30, 2022 · PreprintNeuroscience

Haploinsufficiency of the mouse Tshz3 gene leads to kidney dysfunction

Irene Sanchez-Martin, Pedro Magalhães, Parisa Ranjzad, Ahmed Fatmi, Fabrice Richard, Thien‐Phong Vu Manh, Andrew J. Saurin, Guylène Feuillet, Colette Denis, Adrian S. Woolf, Joost Peter Schanstra, Petra Zürbig, Xavier Caubit, Laurent Fasano

This study shows that Tshz3 haploinsufficiency in mice causes kidney structural, molecular, and functional abnormalities.

Human Molecular Genetics · Dec 15, 2021Peer-reviewedGenetics

A novel mutation (E83Q) unlocks the pathogenicity of human alpha-synuclein fibrils and recapitulates its pathological diversity

Senthil T. Kumar, Anne‐Laure Mahul‐Mellier, Ramanath Narayana Hegde, Rani Moons, Pedro Magalhães, Alain Ibáñez de Opakua, Gwladys Rivière, Iman Rostami, Sonia Donzelli, Markus Zweckstetter, Frank Sobott, Hilal Ahmed Lashuel

The paper investigates the effects of the E83Q mutation on alpha-synuclein aggregation and toxicity, finding it accelerates fibrillization and enhances pathological features in neuronal models.

bioRxiv · Nov 21, 2021 · PreprintNeuroscience