Clemens F. Kaminski

MitoDecode assesses mitochondrial stress types and severity from fluorescence images without segmentation, showing adaptability across cell types and stress conditions.

The paper studies subcellular organization and dynamics in Antarctic fish cell cultures, finding conserved organelle structures but differences in lysosomal size and mitochondrial morphology that may relate to cold adaptation.

This study shows α-synuclein modulates spontaneous dopamine release via calcium and phosphorylation-dependent mechanisms, independent of full-fusion recycling.

The paper develops a hybrid machine learning framework to identify TDP-43 aggregation inhibitors, discovering two compounds effective in reducing aggregation in cell and model organism assays.

The paper introduces a fibre-based structured illumination microscopy (SIM) system that simplifies setup and improves resolution without specialized optics.

This paper introduces an enhanced mountain climbing search algorithm for fast and accurate autofocus in high-resolution fluorescence microscopy, achieving up to 200-fold improvement in precision over existing methods.

FLIMngo, a deep learning model, enables fast and accurate fluorescence lifetime predictions from low-photon FLIM data, improving throughput and reducing phototoxicity for in vivo applications.

This study shows that 24S-HC and 27-HC, brain cholesterol metabolites, cause neuronal dysfunction in human iPSC-derived neurons by disrupting calcium signaling, organelle function, and alpha-synuclein accumulation.

FLIMPA is an open-source software for FLIM phasor analysis that integrates data from multiple trials and provides tools for quantifying microtubule depolymerisation.

This paper develops a high-resolution microscopy method for imaging live biological samples at sub-zero temperatures to study cold-adapted species under physiological conditions.

This paper demonstrates the localization of nucleic-acid cleaving enzyme activity within DNA-based membrane-less compartments, enabling spatially organized enzymatic reactions and dynamic pattern formation.

The paper investigates how mutations in the FUS protein affect axonal cytoskeletal organisation in ALS/FTD models, finding reduced actin density and axonal branch complexity.

The paper introduces PseudoSort, a machine learning algorithm for spike sorting, and applies it to MEA data from hippocampal neurons exposed to monomeric Tau, revealing its impact on neuronal activity.

This study investigates how monomeric and fibrillar alpha-synuclein interact with synaptic vesicles, showing fibrils disintegrate vesicles and increase neuronal uptake, potentially contributing to Parkinson's disease pathology.

The paper develops a multiplex reverse genetics platform to identify genetic modifiers of protein misfolding in neurodegenerative diseases and identifies DNAJB6 as a modifier of toxicity and solubility of ALS/FTD-linked RNA-binding proteins.

The paper develops a unified in vitro to in vivo fluorescence lifetime screening platform to identify amyloid β aggregation inhibitors, revealing a new compound effective in multiple models.

This paper introduces a label-free method using intrinsic fluorescence lifetime to characterize amyloids and their polymorphs, showing distinct fluorescence lifetimes for different amyloid structures and polymorphic populations.

The paper designs binary 2D protein co-assemblies that form ordered, biologically active materials with potential therapeutic applications.

This study shows that intra-mitochondrial proteostasis influences alpha-synuclein and Aβ42 aggregation, while mitochondrial stress factors like complex I inhibition do not affect these pathologies.

This paper introduces a waveguide-based TIRFM system for live-cell imaging of delicate neurons over large fields of view, demonstrating its use with fibroblasts, rat hippocampal neurons, and Xenopus retinal ganglion cells.

The paper shows that ribosomal proteins are synthesized locally in axons and incorporated into ribosomes in a nucleolus-independent manner, regulated by a novel RNA motif.

The paper shows that receptor-ribosome coupling enables selective mRNA translation in axons in response to guidance cues, with receptor-specific interactomes mediating this process.

The paper shows that multipotent progenitors separate specialized stem zones in chick embryos through extrinsic morphogenetic cues rather than intrinsic fate determination.