Wen Xue

Multiplexed cytokine and antigen mRNA administration generates durable anti-tumor immunity against pancreatic cancer

This study shows that delivering multiple cytokine and antigen mRNAs via nanoparticles can generate strong anti-tumor immunity against pancreatic cancer in mice.

Prime assembly with linear DNA donors enables large genomic insertions

This paper introduces a Prime Assembly method for inserting large DNA fragments into the genome, achieving efficient and precise insertions up to 11 kilobase pairs.

KRAS withdrawal in Cholangiocarcinoma leads to immune infiltration and tumor regression

This study shows that removing KRASG12D in a mouse model of cholangiocarcinoma leads to tumor regression through immune infiltration and activation of CD8+ T cells.

A Compact Base Editor Rescues AATD-associated Liver and Lung Disease in Mouse Models

This paper demonstrates that a compact ABE corrects the PiZ mutation in AATD mouse models, reducing liver disease and improving lung function.

Adenine Base Editing in vivo with a Single Adeno-Associated Virus Vector

This study develops a compact adenine base editor (Nme2-ABE) that can be delivered via a single AAV vector to efficiently edit mouse genomic loci and revert disease mutations in a tyrosinemia model.

Precision Cas9 Genome Editing in vivo with All-in-one, Self-targeting AAV Vectors

This paper develops single-AAV vectors for precise in vivo genome editing using Nme2Cas9 with HDR templates or multiple sgRNAs, showing efficacy in treating HT-I and MPS-I mouse models.

Development of a flexible split prime editor using truncated reverse transcriptase

This study develops a compact prime editor with reduced reverse transcriptase activity, showing efficient editing and enabling flexible split-PE design for in vivo delivery.

All-in-One Adeno-associated Virus Delivery and Genome Editing by Neisseria meningitidis Cas9 in vivo

This study introduces NmeCas9 as a new in vivo genome editing tool, demonstrating its effectiveness in targeting specific genes in mice using a single recombinant adeno-associated vector.