Denis L. J. Lafontaine

This study shows mechanical compression remodels the nucleolus, reduces rDNA transcription, and identifies nucleolin as a mediator of this response through biophysical and epigenetic mechanisms.

This study introduces NanoRibolyzer, a long-read nanopore sequencing method to map pre-rRNA processing and modification at single-nucleotide resolution, revealing cleavage sites and modification patterns.

The paper maps rRNA processing dynamics in the nucleolus and shows how rRNA processing affects nucleolar phase organization and structure.

The paper shows that Nopp140 concentrates intrinsically disordered regions to form the DFC, a phase-separated condensate that enables efficient rRNA modification.

This study shows that ZBP1 condensate formation, driven by Z domain binding to Z-RNA, promotes necroptotic cell death to restrict HSV-1 infection.

The paper develops AquIRE to quantify RNA damage and identifies multiple mechanisms of clinically induced RNA modifications, including glycoRNA dynamics across life kingdoms.

The paper uses rRNA modification patterns to identify tissue and tumor-specific signatures, showing their potential as diagnostic biomarkers.

The paper shows mitotic chromosomes are self-entangled and disentangle through a Topoisomerase II-dependent two-stage process during mitotic exit.

The paper tracks heritable methylation transmission in C. elegans and shows that parental starvation induces intergenerational hormesis via 18S rRNA methylation.

This study investigates the role of fibrillarin in ribosome biogenesis and its impact on neural crest cell development in Xenopus laevis, revealing developmental defects and hypomethylation patterns in rRNA.

This study shows that nucleolar stress affects mutant Huntingtin toxicity and progression in Huntington disease by altering rRNA maturation and NPM1 localization.

The paper investigates the role of SNORD13 in rRNA acetylation across eukaryotes, showing its requirement in human and zebrafish but not in yeast or C. elegans.

The paper integrates protein imaging and interaction data to create a unified map of eukaryotic cell structure, revealing new subcellular systems and protein associations.

The paper develops a targeted Nanopore direct rRNA sequencing approach to assess disease-associated rRNA modifications in human cells and tissues.

This study introduces liquid chromatin Hi-C to analyze chromatin interaction dynamics and finds that compartmentalization stability varies with chromatin domain type and fragment size.

The paper identifies DNA methylation as a key mechanism for maintaining monoallelic expression on autosomes and reveals its tunable control of allele-specific expression.

The paper identifies a novel base-pairing interaction in the U8 snoRNA that underlies a Mendelian form of cerebral small vessel disease by analyzing zebrafish models and patient fibroblasts.

The paper identifies a single m1A modification in rRNA that affects ribosome structure and translation of metabolic enzymes in yeast and humans.