Fungal and yeast genetics research

Sugar-induced cell death in exponentially growing yeast depends on the functionality of the nonoxidative branch of the pentose phosphate pathway.

The lipid raft marker flotillin FloA drives relocalization of the plasma membrane H+-ATPase PmaA as a protective response to calcium stress

This paper shows that FloA, a flotillin in Aspergillus nidulans, is required for the internalization of PmaA during calcium stress, preventing calcium overload and promoting cell survival.

Synthetic collinearization of a chromosome segment enables genetic dissection of incompatibility between distant genera

This paper demonstrates that collinearization can overcome genetic incompatibility between distant yeast species by creating synthetic chromosome segments that substitute for native ones, revealing hundreds of genetic incompatibilities.

Universal principles of protein resource allocation among cellular organelles revealed by yeast large-scale absolute quantitative proteomics

This study uses yeast large-scale absolute quantitative proteomics to reveal universal principles of protein resource allocation among cellular organelles and its relationship to physiological parameters and species divergence.

UV-induced translation capacity is regulated developmentally in fungi.

This study shows UV radiation increases ribosome levels and translation capacity in filamentous fungi after filament formation, enhancing UV survival and repair through mechanisms involving TOR signaling and gene expression modules.

A nematode-trapping fungus orchestrates polarity cues, septins, and NOX signaling for trap formation

This study identifies molecular mechanisms coordinating cell polarity, septins, and NOX signaling in nematode-trapping fungi for trap formation.

A membrane-free spot-plating protocol for Agrobacterium-mediated transformation of diverse yeasts

This paper introduces a membrane-free spot-plating protocol for Agrobacterium-mediated transformation of diverse yeasts, improving efficiency and reducing costs.

Endosome motility controls light-responsive reproductive development and secondary metabolite production in Aspergillus

This study shows that endosome motility in Aspergillus species affects light-responsive development and secondary metabolite production.

PAK-family kinases promote cell fusion irreversibility by preventing cell wall repair

This study shows PAK-family kinases prevent cell wall repair after fusion, ensuring irreversibility in fission yeast.

Ectopic overproduction of cell wall glucan through membrane perturbation by an antifungal peptide theonellamide A in fission yeast

This study shows that the antifungal peptide TNM-A causes ectopic overproduction of cell wall glucan in fission yeast by altering Cdc42 activation and glucan synthase localization.

RNA degradation by ribonuclease T2 is required for phosphate homeostasis in S. cerevisiae

The paper shows that RNase T2-mediated RNA degradation is essential for maintaining phosphate homeostasis in S. cerevisiae, even under phosphate-replete conditions.

Synthetic lethality of fungal catabolite repressors reveals essential coupling of carbon repression with mitochondrial and ribosomal gene expression

This study identifies Mig3 as a redundant CCR repressor in fungi, showing its essential role in regulating mitochondrial and ribosomal gene expression alongside Mig1.

Single-cell imaging reveals a key role of Bck2 in budding yeast cell size adaptation to nutrient challenges

The paper uses single-cell imaging and machine learning to show Bck2 is critical for yeast cell size adaptation to nutrient changes, unlike in steady-state conditions.

Oxidative Stress-Responsive Cell Wall Remodeling Depends on Phosphate in Candida albicans

This study shows that phosphate availability influences cell wall remodeling in C. albicans under oxidative stress, linking nutrient sensing to fungal mechanical resilience.

Organelle scaling over a 100-fold cell size range

The paper examines how organelle content scales with cell volume in a fungus with a large size range, finding that some organelles scale with volume while others do not.

Organelle partitioning in the multi-budding yeast Aureobasidium pullulans

The paper studies organelle inheritance in the multi-budding yeast Aureobasidium pullulans, finding that some organelles are evenly distributed while others are variably inherited.

Carbohydrate utilization regulator reveals a noncanonical mechanism of nutrient differentiation

The paper identifies a transcription factor in Rhodotorula toruloides that specifically inhibits glucose-mediated repression of disaccharide utilization, revealing a tailored carbon catabolite repression mechanism.

Cell wall forming chitin synthases in a chytrid fungus

This study identifies and characterizes chitin synthase genes in the chytrid fungus Rhizoclosmatium globosum, revealing their expression patterns and localization during development.

Evolutionary diversification of invertase paralogs couples carbon metabolism and sexual reproduction in fission yeasts

This study identifies evolutionary patterns of gene gain and loss in fission yeasts, revealing how metabolic genes contribute to diversification and link carbon metabolism to sexual reproduction.

Predictive models of the genetic bases underlying budding yeast fitness in multiple environments

The paper uses machine learning to predict yeast fitness across 35 environments using genetic variants, finding that copy number variants explain most fitness variation and that gene contributions depend on isolate and environment.

High-variance phenome database reveals important roles of WD40 proteins in the plant pathogenic fungus Fusarium graminearum

The paper identifies 21 core WD40 genes in Fusarium graminearum through phenome analysis of deletion mutants, linking them to virulence and sexual reproduction.

Gene expression is stable despite widespread cis and trans regulatory divergence in Saccharomyces yeasts

The paper examines regulatory divergence in Saccharomyces yeasts and finds that while cis and trans regulatory effects are widespread, hybrid gene expression remains largely conserved due to compensatory divergence.

Genetic determinants that permit growth without the core septin Cdc12 in Cryptococcus neoformans

The paper identifies genetic factors that allow Cryptococcus neoformans to grow without the core septin Cdc12, revealing compensatory mechanisms for septin-independent cell integrity.

A fungal hemophore relay mediates heme transfer via transient protein interactions

This study investigates heme transfer in Candida albicans using fluorescent hemophore fusions, revealing distinct kinetic mechanisms for heme acquisition and transfer between hemophores.

Capture of the SmSTRIPAK proxiome identifies the greenbeard proteins SmDOC1/2 as regulators of the MAK2 pathway to control sexual development in Sordaria macrospora

The paper identifies SmDOC1/2 as regulators of the MAK2 pathway in Sordaria macrospora, showing non-redundant roles in sexual development through proximity mapping and genetic analysis.

A Glucan Synthase-Remodeler Module Organizes Branched Glucan Assembly in the Fungal Cell Wall

This study identifies Ghs2 as a binding partner of Bgs3 in fission yeast, showing they co-localize and work together to synthesize and branch β-1,3-glucan in the fungal cell wall.

A haploid wild yeast resource for exploring the natural ecology of Saccharomyces cerevisiae

This paper establishes a haploid yeast strain collection to study ecological and reproductive divergence in wild S. cerevisiae, finding that structural genomic variation and metabolic gene divergence drive these traits.

Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens

The paper expands and optimizes the AID2 system for use in Candida species, including clinical isolates and C. auris, enhancing its utility for protein function studies.

Repurposing native non-homologous end joining for multicopy random integration in Wickerhamomyces ciferrii

This paper repurposes the native non-homologous end joining (NHEJ) bias in Wickerhamomyces ciferrii to create a multicopy genome integration platform for metabolic engineering.

Experimental design and analysis of sulfide-induced invasive growth in wine yeast

This study identifies conditions for observing sulfide-induced invasive growth in wine yeast and shows genetic and environmental factors influence this behavior.