Advanced Fluorescence Microscopy Techniques

This paper introduces a real-time 3D single-particle tracking technique combining localization and fluorescence lifetime measurement using a SPAD array detector, achieving 30 nm precision and microsecond resolution.

This paper demonstrates non-degenerate two-photon imaging in rodent cortex using indocyanine green in the water absorption window, enabling deeper vascular imaging.

This paper introduces FlexSIM, a SIM reconstruction method that handles imaging challenges and achieves state-of-the-art performance on open datasets.

PUFFFIN labels cell neighbourhoods using a fluorescent protein fusion, enabling tracking of cell interactions and profiling non-cell-autonomous responses in mammalian cells.

The paper combines TIRF and structured illumination microscopy to enable quantitative single-molecule analysis and track receptor dynamics in the plasma membrane.

The paper introduces iMAX FRET, a single-molecule method for structural analysis that captures 3D biomolecular structures and conformational changes through FRET and geometrical reconstruction.

This paper introduces phalloidin-based single-molecule labeling and localization microscopy (SMLLM) for superresolution imaging of F-actin, showing improved quantification and structural preservation in dendritic cells.

The paper introduces a 3D optical mesoscopy method to quantify GLUT4 levels in intact mouse hearts and shows gender-dependent expression changes with high fat diet.

The paper introduces self-quenched fluorophore-DNA labels to reduce background signal in super-resolution microscopy, improving imaging performance in STED and SMLM.

This paper introduces a high-speed two-photon microscope using adaptive line-excitation to image neural activity in mouse cortex, increasing imaging speed and reducing laser power.

The paper introduces an improved BiFC method for validating protein interactions in plants by incorporating bicistronic expression and self-cleaving peptides to better assess expression and interaction status.

This paper detects fluorescent protein mechanical switching in living cells using FRET-based biosensors and shows it is reversible and influenced by cellular forces.

The paper develops a new fluorophore, NOSR, that enables controlled single-molecule blinking for improved super-resolution imaging of microtubules and mitochondrial outer membranes.

This paper introduces a TCSPC-based flow cytometer for real-time single-cell autofluorescence lifetime analysis, demonstrating viability and sensitivity to metabolic changes in various cell types.

This paper introduces an adaptive optical third-harmonic generation microscope that improves in vivo imaging by correcting optical aberrations in complex tissues.

This paper introduces a miniaturized mesoscope (SOMM) for high-resolution calcium imaging in freely moving mice, achieving widefield, cellular resolution with robustness against motion artifacts.

This paper introduces FAST tags that modulate fluorescence lifetime to enable multiplexed imaging of up to six targets in live cells and zebrafish embryos.

This paper introduces a split fluorescent protein system for tissue-specific and endogenous protein labeling in zebrafish, demonstrating its use for labeling cytoskeleton genes and manipulating protein function.

The paper improves fluorescent antibody labeling to enhance signal intensity for multiplex flow cytometry, showing internal labeling increases brightness and enables triple staining.

The paper describes a widefield in vivo imaging system for mesoscopic brain imaging, capable of capturing fluorescent and reflectance signals, and demonstrates its use in awake mice.

This paper introduces a new photoactivatable blue fluorescent protein (PA-BFP) created through a mutation in sumireF, suitable for use in fluorescence microscopy.

This study compares two FLIM microscopes and finds that differences in cAMP kinetics are due to phototoxicity from FluoroBrite medium, not the imaging technique itself.

This paper introduces a novel optical design for axial de-scanning in light-sheet microscopy's detection arm, enabling aberration-free, multi-color volumetric imaging without sample translation.

The paper correlates calcium multiphoton imaging with STED imaging of the slit diaphragm in the same glomerulus to study kidney disease mechanisms.

This paper introduces a microscope combining targeted illumination and confocal gating to improve voltage imaging in dense tissues, enhancing signal-to-noise ratio and reducing crosstalk.

This paper introduces uiPSF, a toolbox to infer accurate PSF models from microscopy data for improved SMLM imaging and microscope characterization.

This paper introduces StayGold fluorescent protein fusions to enable long-duration, high-speed 3D single-virus tracking in live cells.

The paper develops a plasmid vector system for cloning and expressing genes with fluorescent tags in Haloferax volcanii, enabling functional studies of protein fusions.

This paper introduces HIF-Clear, a method for clearing and labeling FFPE brain specimens, enabling analysis of neuronal circuitry in whole brains.

The paper introduces a magnetic voluntary head-fixation system for longitudinal imaging of hippocampal neurons in transgenic rats.