Force Microscopy Techniques and Applications

This paper uses scanning probe microscopy to study the mechanical properties of biomolecular condensates, revealing their gelation and rejuvenation processes.

This paper uses high-speed in-line force mapping to study dynamic mechanical properties of living E. coli during division, revealing transient phenomena like localized stiffening and membrane pore formation.

This paper introduces a hermetically sealed AFM sample chamber for safe, high-resolution time-lapse imaging of pathogenic microorganisms in vitro.

This paper introduces a Co2+-mediated adsorption method for DNA on mica to improve AFM imaging resolution by reducing salt precipitation compared to Ni2+.

This paper describes a surface passivation assay to reduce non-specific adsorption for single-molecule studies of chromatin and topoisomerase II.

This study uses molecular dynamics simulations to show how potassium and magnesium ions affect nucleosome binding to mica surfaces, with potassium allowing stable binding with less structural change.

The paper investigates how anchor point selection affects the mechanical stability of an Affibody/PD-L1 complex using multiple experimental and computational methods.

This paper introduces a drift-invariant force calibration method for multiplexed magnetic tweezers using Hadamard variance, showing higher precision and accuracy than existing techniques.

This paper introduces a single-molecule stretching assay to detect DNA-intercalating agents from microorganisms, identifying two strains and three compounds with anti-cancer activity.

The paper investigates the mechanical strength of ClfA, a bacterial adhesin, and finds it is not inherently strong without its human target.

This study uses optical nanotweezers to investigate how ascorbic acid and pH affect single ferritin disassembly, identifying key fragments and cooperative behavior.

This paper introduces DNA-based ForceChrono probes to measure single-molecule force dynamics in living cells, revealing precise loading rates and a link between force magnitude and duration.

The paper introduces COMBI-Tweez, a tool combining optical and magnetic tweezers with fluorescence microscopy to study the structural dynamics of single chiral biopolymers like DNA under mechanical forces.

NanoLocz provides an open-source platform for automated analysis of AFM, high-speed AFM, and LAFM data, enabling single-particle tracking and other workflows.

The paper introduces a GPU-accelerated atom filtering algorithm to improve the efficiency of simulated atomic force microscopy (AFM) image calculations for biomolecular structures.

This study uses high-resolution AFM and image analysis to analyze the topology of kinetoplast DNA, revealing structural properties and changes upon digestion.

The paper describes an AFM force mapping pipeline to analyze mechanical properties of cryosectioned soft tissues, showing that repeated measurements, outlier exclusion, and log-normal transformation improve reliability.

The paper measures the mechanical properties of NompC gating springs and finds nonlinear compliance that may regulate mechanotransduction sensitivity.

This study demonstrates a method using magnetic tweezers to detect single base pair mismatches in DNA by observing plectoneme pinning under specific conditions.