Biosensors and Analytical Detection

Duplex Reverse Transcription Loop-Mediated Isothermal Amplification on a Nanofluidic Digital Chip (Nano-dChip)

The paper introduces a nanofluidic digital chip for rapid, low-cost viral RNA detection with high sensitivity and specificity, demonstrating its effectiveness for SARS-CoV-2 and Influenza H3.

B-Lactamase-Responsive Probe for Rapid and Non-invasive Detection of Tuberculosis through Induced Breath Analysis

This paper develops a breath analysis method using a beta-lactamase-responsive probe to detect tuberculosis rapidly and non-invasively.

Fast and sensitive multiplexed diagnostic system enabled by real-time solid-phase PCR assay

This paper introduces a flow-through solid-phase PCR platform for rapid, sensitive, and quantitative detection of multiple viral pathogens in a single reaction.

Highly sensitive enzyme- and amplification-free, quantitative DNA detection using YVO4:Eu luminescent nanoparticle probes

This paper develops a sensitive, amplification-free DNA detection method using YVO4:Eu nanoparticles, achieving 50 aM sensitivity for SARS-CoV-2 n1 gene detection.

You Only Look Once (YOLO) Based Machine Learning Algorithm for Real-Time Detection of Loop-Mediated Isothermal Amplification (LAMP) Diagnostics

This paper develops a YOLO-based algorithm to automate LAMP result classification, achieving high accuracy and precision in detecting positive and negative cases.

Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization

This paper develops a low-cost, high-throughput multiplexed cytokine detection platform for TB diagnostics using amplified enzymatic silver metallization and cellphone imaging.

CRISPR/Cas14a Combined with RPA for Visual Detection of Marek's Disease Virus

The paper develops a rapid, instrument-free, and specific nucleic acid detection method for Marek's Disease Virus (MDV-1) using CRISPR/Cas14a and RPA, achieving a detection limit of 24.6 copies/L and 100% accuracy in clinical samples.

Instrument-free quantitative detection of analytes using a novel combo lateral flow assay

This paper introduces a combo lateral flow assay (CLFA) that enables quantitative detection of analytes without external instruments, using AI-based image analysis for improved precision.

Detection of five viruses commonly implicated with Bovine Respiratory Disease using loop-mediated isothermal amplification

The paper develops qLAMP and RT-qLAMP assays for detecting five BRD-associated viruses with high sensitivity and specificity, and introduces a streamlined pipeline for assay development.

A novel approach for the extraction of nucleic acids using a hybrid paper-plastic device

This paper develops a low-cost, equipment-free paper-plastic cassette for nucleic acid extraction, showing comparable results to commercial kits and suitable for point-of-care diagnostics.

Lab on a Capillary: Instrument-Free Compartmentalization Using Photopatterned Hydrogel Rings Embedded inside a Glass Capillary for Amplified Bioassays

The paper introduces LabCap, a low-cost, instrument-free platform for biomarker detection using hydrogel rings in glass capillaries, achieving sensitivity down to 0.1 ng/mL for CRP and NT-proBNP.

DISCO-LAMP: A Novel discontinuous LAMP assay for isothermal antigen detection

The paper introduces DISCO-LAMP, a novel LAMP assay for isothermal antigen detection, achieving high sensitivity and low background amplification.

A solid-state heater-imager for quantitative evaluation of colorimetric isothermal nucleic acid amplification on paper

The paper introduces a dual-sided heater-imager system for quantitative colorimetric isothermal nucleic acid amplification on paper, achieving temperature uniformity and real-time analysis.

Development of a point-of-care field diagnostic test for DFT1 and DFT2

This paper develops a field-deployable diagnostic test for DFT1 and DFT2 using SHERLOCK to detect DNA in attomolar range and non-invasive samples.

Silicon Micropillar-Enhanced CRISPR Biosensor for Rapid and Sensitive Detection of Drug-Resistant Bacteria

This paper develops a silicon micropillar-enhanced CRISPR biosensor for detecting drug-resistant bacteria, achieving high sensitivity and specificity for MRSA.

Microfluidic Immunocapture Device for Direct Detection of Lyme Disease

This paper introduces a microfluidic immunocapture device for direct detection of Lyme Disease by capturing Borrelia organisms, improving detection accuracy over traditional antibody-based methods.

NFC-enabled Sensing Platform for the Onsite Determination of Asparagine in Food

The paper develops an NFC-enabled sensor for rapid, cost-effective detection of free asparagine in food to reduce acrylamide formation.

Affinity-based selection of anti-Feline Leukaemia Virus p27 monoclonal antibodies for efficient lateral flow assay development

This study develops an efficient method to select monoclonal antibodies for lateral flow assays by combining ELISA and Spectral Shift, enabling rapid and resource-efficient antibody screening for diagnostic applications.

Development of Loop Mediated Isothermal Amplification for Rapid and Sensitive Detection of Enteroaggregative Escherichia coli (EAEC).

This study develops a LAMP assay for rapid, sensitive detection of EAEC with higher sensitivity than conventional PCR.

SIMAIS: A swarm intelligence inspired biosensor for rapid protein detection

SIMAIS uses swarm intelligence to create a rapid, sensitive biosensor for protein detection, achieving tenfold higher sensitivity than conventional assays in 10 minutes.

Mechanistic Evaluation of Amplification Lag in Paper-Based Colorimetric Loop Mediated Isothermal Amplification (LAMP) and Its Reduction by BSA Pre-Coating

The paper evaluates amplification lag in paper-based LAMP assays and shows BSA pre-coating reduces it by improving diffusion and reducing adsorption.

Lateral flow cell-free transcriptional assay for contaminant detection

This paper develops a highly sensitive lateral flow assay for detecting metal contaminants, achieving regulatory limits for lead in environmental samples.

Machine Vision Enabled Lateral Flow Immunoassay Using Functionalized Gold Nanoparticles for Point-of-Care Cardiac Biomarker Detection

This paper develops a machine vision-enhanced lateral flow immunoassay using functionalized gold nanoparticles for rapid, sensitive detection of cardiac biomarkers cTnI and Myo in point-of-care settings.

Electrochemical Lateral Flow Assay with Linked Analytics for the Surveillance of Cassava Brown Streak Disease in East Africa

This paper introduces ELLA, a low-cost diagnostic tool for CBSD surveillance, achieving high accuracy and enabling scalable plant disease monitoring through electrochemical sensing and AI.

A novel tripod probe and lateral flow test to improve CRISPR/Cas12a assay: benefits of branched probe based on trebler phosphoramidite modification

This paper introduces a CRISPR/Cas12a-based assay with a tripod probe and lateral flow test that improves sensitivity and signal-to-noise ratio for nucleic acid detection.

Antibody-Initiated Loop-Mediated Isothermal Nucleic Acid Amplification (ai-LAMP) as a New Biosensor for Antigen Detection

This paper develops ai-LAMP, a biosensor platform for sensitive protein detection using antibody-initiated loop-mediated isothermal amplification, achieving a 46x improvement in sensitivity for HIV-1 p24 detection compared to existing methods.

A Portable RPA-CRISPR/Cas12a-Based Biosensing Platform for On-Site Detection of the Microcystin Synthetase E Gene in Lake Water

This paper develops a portable RPA-CRISPR/Cas12a biosensing platform for rapid on-site detection of the microcystin synthetase E gene in lake water with high sensitivity and field applicability.

A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection

The paper introduces RAMONA, a rapid, modular nanobody assay for antigen detection that combines portability with high sensitivity and adaptability for diverse targets.

Surface-Enhanced Raman Spectroscopy-Assisted Lateral Flow Test for Adenine and IgG Analysis

This paper develops a lateral flow test using surface-enhanced Raman spectroscopy for detecting adenine and IgG, improving selectivity and sensitivity in complex samples.

A handheld microfluidic manifold for massively multiplexed nucleic acid detection

This paper introduces a portable microfluidic device for highly multiplexed nucleic acid detection, demonstrating its integration with CRISPR-based technology for rapid pathogen and variant detection.