Biosensors and Analytical Detection

AI-guided discovery of natural pocket proteins for portable small-molecule sensing

The paper develops an AI-guided workflow to identify natural pocket proteins for small-molecule sensing, achieving 43.8% binding response in tested pairs and demonstrating portable device performance with low serum LODs.

Rapid RPA-CRISPR/Cas12a detection of Vibrio coralliilyticus for coral aquaculture disease surveillance

This paper develops a rapid diagnostic assay for Vibrio coralliilyticus using RPA-CRISPR/Cas12a, showing high specificity and sensitivity for coral disease surveillance.

Development and Analytical Validation of a Smartphone-Based Quantitative Lateral Flow Immunoassay for Serum Cystatin-C

This paper develops and validates a smartphone-based lateral flow immunoassay for serum Cystatin-C, showing high accuracy and reliability in clinical samples.

Bispecific Coiled-Coil Reporters Enable Rapid, Multiplexed Detection of Mycoplasma via Toehold Switch Sensors

This paper introduces BINOCULAR, a system for rapid, multiplexed detection of Mycoplasma using toehold switch sensors, achieving sub-5-minute readout times and 1-copy/µL sensitivity.

Surfactant-Assisted Colorimetric Signal Enhancement in Paper-Based Glucose Sensing

This study identifies Tween 20 as a surfactant that enhances colorimetric glucose sensing on paper by preserving enzyme activity during drying, improving detection limits and signal stability.

Base-mismatched crRNA in CRISPR Enabled Universal One-pot Rapid Nucleic Acid Detection

This paper introduces a one-pot CRISPR-Cas12a diagnostic strategy using base-mismatched crRNA, enabling rapid and specific detection of pathogens without PAM re-design or costly modifications.

A rapid, field-deployable paper-based biosensor for the detection of African swine fever virus in whole blood

This paper develops a portable biosensor for rapid ASFV detection in whole blood using microfluidic paper-based devices, achieving high sensitivity and specificity without DNA extraction.

Artificial Intelligence-Driven Nanosensing to Identify Circulating Bacterial DNA: A Novel Approach to Breast Cancer Risk Profiling

This paper develops a PQD-based nanosensor using LSTM deep learning to detect bacterial species linked to breast cancer, showing high sensitivity and selectivity for early detection.

Extrusion-Printed Silicone Microarchitectures for Geometry-Controlled Flow in Lateral Flow Diagnostics and Paper Microfluidics

This paper develops a silicone ink for high-resolution hydrophobic barriers on paper, improving fluid control and diagnostic sensitivity in paper-based diagnostics.

Multi-feature Classification to Improve Colorimetric Loop-Mediated Isothermal Amplification Fidelity

The paper develops a machine learning model to improve LAMP assay design by identifying key thermodynamic features from primer sets, achieving high classification accuracy.

Attomolar Detection of HIV-1 with Label-Free RCA-rCRISPR on Smartphone

This paper develops a label-free diagnostic platform for HIV detection with smartphone integration, achieving attomolar sensitivity without sample pre-treatment.

A rapidly deployable CRISPR-Cas3 diagnostic platform for emerging RNA viruses

This paper develops a CRISPR-Cas3-based diagnostic platform for emerging RNA viruses, enabling rapid assay design and deployment with high sensitivity and portability.

Artificial Intelligence of Things-Enhanced Automated Surveillance System for Global Antimicrobial Resistance in Food Supply Chain

This paper develops an AIoT-integrated system for automated AMR surveillance in food supply chains, achieving high accuracy and cost reduction.

A Portable Fluorescence Platform for Decentralized One-Health mcr-1 Monitoring

The paper develops a portable fluorescence platform for decentralized monitoring of mcr-1, achieving high sensitivity and concordance with sequencing and phenotypic tests.

Semi-quantitative Classification of HIV-1 Nucleic Acids Using ResNet Image Analysis of Discretized Isothermal Amplification Reactions in a Microfluidic Chip

This paper develops a machine learning approach to semi-quantitatively classify HIV-1 nucleic acids using image analysis of isothermal amplification reactions in microfluidic chips, achieving high accuracy across a wide concentration range.

Smartphone-integrated RPA-CRISPR-Cas12a Detection System with Microneedle Sampling for Point-of-Care Diagnosis of Potato Late Blight in Early Stage

This paper develops a portable smartphone-integrated RPA-CRISPR-Cas12a system for early detection of potato late blight using microneedle sampling, achieving high sensitivity and field usability.

Embedded transport accelerates interaction-limited biosensing

This paper introduces a hydrogel-based biosensor that accelerates bacterial interaction with reporter molecules through engineered transport, achieving faster and more accurate detection of infections and antimicrobial susceptibility.

Fieldable isothermal nucleic acid test for rapid semi-quantitative visual readout of Enterococci in recreational waters

The paper introduces a rapid, semi-quantitative LAMP-OSD test for detecting Enterococci in water, enabling visual readout and comparison with contamination standards.

Transforming off-the-shelf personal glucose meter into a sustainable and decentralized label-free nucleic acid and NAAT detection platform

This paper repurposes commercial glucometers to detect nucleic acids and NAAT products using a redox-mediated mechanism, enabling low-cost, portable, and sustainable point-of-care testing.

Light-Activated Nucleic Acid Amplification Systems Using Photo-caged DNA Polymerase or Primers

This paper develops light-activated RAA systems using photocaged DNA polymerase or primers to enable precise, spatiotemporal control of nucleic acid amplification for field-ready diagnostics.

Ultrasensitive, Low-Input Detection of Avocado Sunblotch Viroid via RPA-CRISPR and Nanopore-Array Single-Bead Fluorescence Readout

This paper develops a low-input, ultrasensitive diagnostic platform for detecting Avocado Sunblotch Viroid using RPA-CRISPR and nanopore-array fluorescence readout, achieving a detection limit of 1.68 copies/L.

Rapid Cas13a-based penA genotyping for cefixime susceptibility in Neisseria gonorrhoeae

This paper develops a CRISPR-based assay for rapid detection of cefixime resistance in Neisseria gonorrhoeae by analyzing penA gene mosaicism.

Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene

This paper optimizes a PCR-CRISPR-Cas12a assay for rapid detection of the blaOXA-1 gene, achieving results in under an hour with basic equipment.

ThermiQuant™ AquaStream: A portable instrument for quantitative colorimetric isothermal nucleic acid amplification reactions in paper and tube formats

ThermiQuant AquaStream is a portable device that enables real-time colorimetric LAMP assays in both microPADs and tubes with high sensitivity and precision.

ThermiQuant(TM) MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests

Machine-learning strategy for high error tolerance in image-based digital molecular assays

This study develops a machine-learning strategy to improve error tolerance in image-based nanoparticle immunoassays for field-based diagnostics.

Development and evaluation of a pan-fungal lateral flow device for the rapid identification of pathogen class in microbial keratitis

This paper develops and evaluates a pan-fungal lateral flow device for rapid identification of pathogens in microbial keratitis using ex vivo porcine cornea models.

Morphology-Guided Deep Learning for Nanoparticle Agglomeration Diagnostic Assays

This paper presents a morphology-guided deep learning platform for nanoparticle agglomeration assays, achieving high sensitivity and recall for DNA and SARS-CoV-2 RNA detection.

Extending the limits of 3D printed polymers on paper towards bioanalytical sensing

This paper develops 3D printed hydrophobic barriers on paper for microfluidic biosensing, identifying polypropylene as the optimal material for creating functional microchannels.

Rapid identification of African swine fever virus in diagnostic samples using CRISPR-Cas

This paper develops a CRISPR-Cas12a assay for rapid, sensitive, and specific detection of African Swine Fever Virus in diagnostic samples, achieving high sensitivity and specificity comparable to qPCR.