Discover, rate, and verify biology preprints.

SEME enables programmable transcript reconstruction through synthetic microexon incorporation

SEME corrects aberrant splicing in iPSC-CMs by programmable microexon incorporation, restoring filamin C expression in a dilated cardiomyopathy model.

Probabilistic model discovery reveals distinct constitutive behavior of kidney cortex and medulla

This paper develops probabilistic strain energy models for kidney cortex and medulla using neural networks, revealing distinct mechanical properties and variability.

Inertial coupling between swing and stance legs shapes ankle torque profile during running

This paper extends the SLIP model to account for swing-leg inertia, explaining the ankle torque profile during running.

A novel route for valerolactam production in Corynebacterium glutamicum: metabolic engineering and bioprocess optimization

This study engineers Corynebacterium glutamicum to produce valerolactam using metabolic pathway modifications and process optimization, achieving a titer of 3.6 g/L with reduced by-product formation.

Representing Sex in Cardiovascular Models: Calibrating Reference Parameters from Healthy Cohorts

This study calibrates reduced-order cardiovascular models using healthy male and female cohorts to establish sex-specific parameter ranges for more inclusive cardiovascular simulations.

Single-cell DNA cytometry with magnetic- and fluorescence-activated bead sorting

The paper introduces magniFIND-seq, a method for single-cell DNA and RNA analysis using digital PCR and commercial sorting instruments, enabling high-purity isolation of rare cell populations.

Accelerated discovery of thermostable vaccines using data-efficient AI

This study uses AI to rapidly develop thermostable mRNA vaccines that maintain bioactivity at high temperatures and can be delivered via microneedle patches.

Engineered nanosponges mitigate peripheral stress-induced neuroinflammation and restore cognitive function

This study develops HA Tau nanosponges that reduce systemic inflammation and cognitive decline in aged mice by sequestering inflammatory proteins without suppressing immunity.

Electrophysiological and neuroimmune responses of a cortical tissue model to chronic gamma radiation exposure

This study examines the effects of acute versus chronic gamma radiation exposure on cortical tissue models, finding preserved neuronal function but glial sensitivity.

Quantitative Gait Analysis in a Sheep Model of In-Utero Spina Bifida Repair

This study evaluates quantitative gait analysis in a sheep model of in-utero spina bifida repair, finding significant differences in gait parameters and classification accuracy between treated and control groups.

Impact of Adipogenic Differentiation on the Nutrition and Sensory Profile of Cultivated Pork Fat Tissue

This study examines how adipogenic differentiation affects the flavor and nutritional profile of cultivated pork fat, finding increased aromatic compounds without full differentiation.

Curved by Design: Applying Microfluidic Principles for Nonplanar and Planar Suspended Tissue Patterning to the Development of Bladder Models with Tunable Mechanics

The paper develops the STEAM platform for creating suspended 3D bladder models with tunable mechanics and spatial complexity.

Metabolic adaptation to TCA cycle deficiency in a strictly aerobic bacterium

This study shows that adaptive evolution can restore growth in a bacterium with a disrupted TCA cycle by reorganizing central metabolism and improving redox balance.

Split suppression gene drives reveal a design tradeoff between inheritance and reproductive load in Aedes aegypti

This study develops split suppression gene drives in Aedes aegypti, showing that inheritance efficiency and reproductive fitness costs must be balanced for effective population suppression.

Bifunctional methacryloyl-norbornene gelatin chemistry enables tunable bioinks for soft tissue engineering via digital light processing

This paper develops a bifunctional gelatin-based bioink for 3D bioprinting, showing improved tunability and cell viability compared to existing formulations.

Designing Well-Organized Touch-Spun 3D Scaffolds: The Role of Interfiber Spacing in Cell Proliferation

The paper evaluates touch-spun 3D scaffolds with varying interfiber spacing to determine optimal conditions for NIH/3T3-GFP fibroblast growth, finding that 50.61 m spacing maximizes cell viability.

Hygrochastic Seedpods: Engineering Microclimates for Forest Restoration

This paper introduces hygrochastic seedpods that create a timed, measurable microclimate for seeds, accelerating emergence without harming growth.

Ultrastructural and Proteomic Signatures of Mechanoadaptive Fibroblast Remodeling across Microphysiological and Mesoscale Shear Platforms

This study shows sustained fluid shear stress drives a profibrotic phenotypic switch in fibroblasts across different mechanical platforms.

Extended normothermic machine perfusion preserves viability and tissue integrity of ex vivo human intestine segments

This study shows that extended normothermic machine perfusion preserves human intestinal tissue viability and function for up to 72 hours, enabling mechanistic studies of inflammation and personalized therapy testing.

Proprietary Processing of Placental Tissue Allografts Influences Wound Healing Trajectories in a Rodent Excisional Skin Wound Model

This study compares proprietary and conventional processing of placental tissue allografts in a rodent wound model, finding the proprietary method promotes better wound resolution and reduced tissue injury.