David Lee Kaplan

Limb-localized induction of regeneration reshapes brain and amputation-site transcriptional states in adult Xenopus laevis

This study examines how limb regeneration in Xenopus laevis affects brain and amputation-site gene expression, identifying changes in metabolic and immune pathways and cross-species similarities in regenerative responses.

Molecular Engineering of Silk via Fatty Acid Esterification for Water-Stable, Anti-fouling, Biocompatible, Implantable Materials

This study modifies silk fibroin with fatty acid esterification to create water-stable, anti-fouling, biocompatible materials for implantable applications.

Fast structural search for classification of gut bacterial mucin O-glycan degrading enzymes

This paper introduces DEFT, a method that combines sequence- and structure-based approaches to improve EC number prediction accuracy and enable high-throughput enzyme repertoire annotation.

Impact of Adipogenic Differentiation on the Nutrition and Sensory Profile of Cultivated Pork Fat Tissue

This study examines how adipogenic differentiation affects the flavor and nutritional profile of cultivated pork fat, finding increased aromatic compounds without full differentiation.

Persistent microbial material contributes to Alzheimer disease and is targetable by vaccination

This study identifies persistent microbial material as a contributor to Alzheimer disease and shows that vaccination targeting it can improve cognition and reduce pathology.

Computational design of blue melanin with peptide motif scaffolding

This paper designs blue melanin using peptide motifs and computational methods, achieving stable blue color with specific absorption wavelengths and thermal/photostability.

Progressive matrix stiffening of tyramine-modified silk fibroin hydrogels governs stage-specific pulmonary fibroblast activation

This study uses stiffening hydrogels to model fibrosis stages and shows how different stiffness levels affect fibroblast activation and cytokine secretion.

Precision Fermentation of Recombinant Myofibrillar Proteins for Future Foods

This study produces recombinant myofibrillar proteins (actin and myosin) via precision fermentation, achieving higher yields and purity than prior methods, and shows improved textural properties when combined with plant-based proteins.

Comparative Transcriptomics of Adherent and Suspension Chicken Fibroblast Cell Lines for the Optimization of Cultivated Meat Processes

This study compares transcriptomic changes in chicken fibroblasts transitioning from adherent to suspension culture, identifying mechanisms that enable suspension proficiency.

Engineered Vibrio natriegens lysate can replace multiple components of cell culture media

This paper shows that engineered Vibrio natriegens lysate can replace fetal bovine serum and fibroblast-like growth factor 2 in cell culture media for cultivated meat, supporting cell proliferation and differentiation.

Multi-modal, Label-free, Optical Mapping of Cellular Metabolic Function and Oxidative Stress in 3D Engineered Brain Tissue Models

This study develops a label-free optical method to assess metabolic function and oxidative stress in 3D brain tissue models using spectral imaging and fluorescence lifetime analysis.

Modulation of Nutritional Composition and Aroma Volatiles in Cultivated Pork Fat by Culture Media Supplementation

This paper shows that adding thiamine-HCl, L-methionine, and myoglobin to cell culture media changes the aroma of cultivated pork fat by increasing specific volatile compounds.

Synergetic hallmark knockouts immortalize bovine muscle stem cells for cellular agriculture

This study knocks out PTEN, TP53, and SMAD4 in bovine satellite cells to create an immortalized cell line that proliferates and differentiates into myotubes, supporting cellular agriculture applications.

Establishment & Characterization of a Non-Adherent Insect Cell Line for Cultivated Meat

This paper establishes and characterizes a non-adherent insect cell line for cultivated meat production, showing high cell density and metabolic profiles.

Microbial lysates as low-cost serum replacements in cellular agriculture media formulation

This study identifies microbial lysates as a low-cost alternative to fetal bovine serum for cultivating meat cells in vitro.

Multi-spectral photoacoustic imaging combined with acoustic radiation force impulse imaging for applications in tissue engineering

This paper introduces a multimodal imaging technique combining ultrasound, photoacoustic, and acoustic radiation force impulse imaging to characterize tissue-engineered scaffolds and monitor cell growth and biomechanical properties.

Chemical and Sensory Analyses of Cultivated Pork Fat Tissue as a Flavor Enhancer for Meat Alternatives

This study compares the sensory and volatile profiles of cultured porcine fat and conventional fat, finding similar aroma characteristics and liking scores.

Decellularized Banana Leaves: Eco-Friendly Scaffolds for Cell-Based Seafood

This study evaluates decellularized banana leaves as eco-friendly scaffolds for cell-based seafood, showing high cell viability and alignment with gelatin coating.

Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model.

This study investigates mitochondrial dysregulation's role in neurodegeneration after contusion injury using a 3D in vitro triculture model of human brain cells.

3D bioengineered neural tissue generated from patient-derived iPSCs develops time-dependent phenotypes and transcriptional features of Alzheimer's disease

Functional maturation of human neural stem cells in a 3D bioengineered brain model enriched with fetal brain matrix

This study shows that using fetal brain matrix in 3D cultures enhances the functional differentiation of human neural stem cells into neurons and astrocytes.