Discover, rate, and verify biology preprints.

Culture enhanced MSCs to boost haematopoietic engraftment in a model of the post-leukaemic bone marrow niche.

This study improves MSCs for HSCT by suppressing senescence and shows enhanced engraftment in an AML niche model.

Impedance-Derived Heart Rate and Heart Rate Variability from tDCS Output Voltage: Sensorless Physiological Monitoring During Electrical Neuromodulation

This paper shows that heart rate, heart rate variability, and respiration can be estimated from tDCS output voltage without additional sensors.

Quantitative evaluation of LED based optical autofocus module

The paper evaluates an LED-based optical autofocus module using 2D autocorrelation analysis and demonstrates its axial stability over time.

Mechanical History and Substrate Stiffness Shape Integrin-Mediated Endothelial Cell Behavior on Bioactive Hydrogels

This study shows that substrate stiffness promotes integrin expression and endothelialization, while mechanical history has a transient effect on cell behavior.

In vivo CRISPR base editing for treatment of Huntington's disease

This study uses CRISPR base editing to create HTT protein isoforms resistant to caspase-6 cleavage, reducing toxicity and neurodegeneration in a rodent HD model.

An Open-Source Magnetofluorescence Imaging Platform forPlate-Scale Screening of Magnetic Field Effects in LiveBacteria

The paper introduces an open-source platform for high-throughput screening of magnetic field effects in live bacteria using magnetofluorescence imaging.

Ultrasensitive Detection of Alpha-Synuclein Oligomers in Human Plasma Using Optimized Nano-QuIC

This study develops an optimized Nano-QuIC platform to detect alpha-synuclein oligomers in human plasma with high sensitivity for early Parkinson's disease diagnosis.

4D Biomimetic Morphing Hydrogel Scaffold via Biaxial Gradient Programming

This paper develops a biaxial gradient-programmed 4D hydrogel system that enables precise, non-uniform shape morphing and supports cell-laden biofabrication for biomimetic tissue engineering.

Bespoke sustainable 3D-printed labware for enhanced handling and standardization of tumor spheroid migration and invasion assays

The paper introduces a 3D-printed platform for standardized tumor spheroid invasion assays, reducing variability and enhancing reproducibility.

A practical workflow for CAR screening in primary T cells using fitness-guided design and mRNA electroporation

The paper presents a workflow for screening CAR variants in primary T cells using fitness-guided design and mRNA electroporation, identifying improved variants with enhanced cytotoxicity.

Functional human neurospheroids recapitulate key features of cortical complexity

This study develops human neurospheroids to model cortical complexity, finding that three-dimensionality and modularity enhance network activity richness and complexity.

Functionalizing hydrogel nanovials with vesicles mimicking antigen-presenting vesicles and cancer exosomes improves T cell capture and activation

Maximizing the productivity of co-transcriptional in vitro transcription

This study optimizes co-transcriptional in vitro transcription (co-IVT) to achieve high mRNA yield and quality, reducing impurities and improving production efficiency for therapeutic applications.

Off-the-Shelf Multilayer Vascular Grafts with Damage-Resistant Hydrogel Coatings Incorporating Integrin Targeting

This paper develops multilayer vascular grafts with damage-resistant hydrogel coatings that promote endothelialization and maintain thromboresistance, showing durability after sterilization and physiological loading.

DigitalPedon: A Novel Digital Twin Framework for Soil Profile Monitoring and Global Soil Data Interoperability

DigitalPedon provides a digital twin framework for soil profiles, addressing interoperability and data gaps in soil science through real-time integration and natural language queries.

Rationally designed split Lettuce aptamer based on large scale mutational analysis

This paper identifies high-performance split sites in DNA aptamers through large-scale mutational analysis, improving signal-to-background ratio for RNA detection.

Variants of relative frequency methods for determining transcranial magnetic stimulation motor threshold do not provide accurate estimation of threshold

This paper shows that variants of relative frequency methods for TMS motor thresholding are inaccurate and slow, recommending instead stochastic approximation or maximum likelihood estimation.

Transplanting enzyme active site geometry into antibody CDRs for catalytic antibody design

This paper presents a computational framework to design catalytic antibodies by transferring enzyme active site geometry into antibody CDRs, enabling controlled catalytic residue arrangement and improved chemical turnover.

BioPathfinder: Evidence-guided multi-agent platform enables hypothesis discovery for CAR-T engineering

A provenance-tracked multi-agent system curated 57 CAR-T patient scRNA-seq studies, generated reviewable hypotheses, and prioritized KLRC1/NKG2A, whose blockade improved CD8+ CAR-T antitumour activity in a Raji xenograft model.

Evaluating research-grade and commercial SFDI platforms for burn severity assessment and feature reduction.

The paper compares research-grade and commercial SFDI platforms for burn severity assessment, finding that dimensionality reduction slightly reduces performance but remains clinically viable.