Discover, rate, and verify biology preprints.

Click chemistry-based quantification of extracellular matrix turnover for drug screening and regenerative medicine

This paper introduces a click chemistry-based method to quantify ECM turnover, validated for drug screening and regenerative medicine applications.

3D Printed Bioelectronic Scaffolds for Impedance-based Cytotoxicity Monitoring of In Vitro Cancer Models

This paper introduces 3D printed bioelectronic scaffolds for impedance-based cytotoxicity monitoring in 3D cancer cell cultures, demonstrating their effectiveness in drug response assessment.

Online characterization of surrogate metrics for metabolic phenotype in human induced pluripotent stem cell bioprocessing

This paper develops an open-source system to monitor metabolic phenotype surrogates in hiPSC bioprocessing using real-time measurements of pH, dissolved oxygen, and cell density.

Genipin-Crosslinked, Silane-Anchored 3D Tumor-Stroma Microtissues for High-Content On-Chip Drug Testing

This paper develops a microfluidic platform for 3D tumor-stroma co-cultures that stabilizes ECM and supports long-term drug testing with high-content imaging and AI analysis.

Membrane-resolved epithelial electrophysiology revealed using extracellular electrochemical impedance spectroscopy (EEIS)

This paper introduces EEIS to measure apical and basolateral membrane properties in epithelial cells, showing it can detect changes in ion permeability and response times in response to drug treatments.

Amphiphilic Peptide Fusion Promotes Endocytic Uptake of Nanodiscs

This study shows that fusing an amphipathic peptide to nanodiscs enhances endocytic uptake and reduces endosomal sequestration, improving drug delivery efficiency.

Probabilistic Cardiac Digital Twins for Robust Patient-Specific Modeling

This paper introduces a framework for probabilistic cardiac digital twins that enables uncertainty quantification through joint probability density learning and conditional regression.

A multiscale modeling approach to study the role of mechanics and inflammation in pathophysiology of articular cartilage

This paper develops a multiscale model linking tissue mechanics, cellular forces, and gene regulation in articular cartilage to study OA pathophysiology.

MSK-Morph: An automated framework to systematically morph landmark-defined musculoskeletal models into subject-specific bone geometries

MSK-Morph automates the transformation of template musculoskeletal models into subject-specific bone geometries using medical imaging data.

Bio-Inspired Ultrasound-Driven Ultrafast Soft Microgripper

This paper develops a bio-inspired ultrasound-driven soft microgripper that enables ultrafast and precise manipulation of delicate objects.

Temporal Structure of Environmental Noise Controls the Localization and Tracking of Populations of Chemotactic Microorganisms

This study shows that the temporal structure of environmental noise, not its amplitude, controls chemotactic population behavior by influencing stability and tracking accuracy.

Discovery and Characterization of Interleukin-4-Specific Affibodies for Affinity-Controlled Protein Release and Macrophage Polarization

This paper develops IL-4-specific affibodies to control cytokine delivery and bioactivity, showing reduced IL-4 release and partial signaling inhibition in macrophages.

Dual Immune Checkpoint and Cytokine Receptor Modulation by an Engineered Human CTLA-4/IL-10 Bispecific Fusion Protein

This paper describes the engineering of a bispecific fusion protein combining CTLA-4 and IL-10 to modulate immune checkpoints and cytokine receptors, showing T cell-inhibitory effects in vitro.

Early electrical stimulation promotes functional recovery after volumetric muscle loss

This study shows that early electrical stimulation improves functional recovery in rats with volumetric muscle loss by enhancing muscle remodeling.

Compact adenine base editors to enable therapeutic rescue of Duchenne muscular dystrophy

This paper develops compact adenine base editors for in vivo gene editing, demonstrating their application in Duchenne muscular dystrophy by enabling exon skipping through targeted splice-site disruption.

Machine Learning-Driven Multiplexed Biomarker Detection with Polymer-Enhanced Electrochemical Sensors

This paper develops a polymer-modified electrochemical sensor array combined with machine learning to accurately detect multiple biomarkers in complex mixtures.

In vivo programming of stem-like CAR T cells by lymphatic-selective lipid nanoparticle enables durable anti-tumour efficacy in orthotopic model

This paper develops a lipid nanoparticle strategy to enhance CAR-T cell persistence and antitumour efficacy in solid tumours by targeting primary lymphoid tissues.

A Tissue Proteolysis Activity Mapping and Substrate Discovery Platform for Identifying Novel Tumor-Activated Biosensors

This paper introduces PSurf, a platform for identifying tissue-specific protease sensors, and demonstrates its use in detecting tumor-specific proteolytic activity in lung tissue.

Modular Albumin-Chaperoned NIR-II Nanofluorophores Enables Pan-Ovarian Cancer Imaging Across Multiscale Tumor Models

This paper develops NIR-II nanofluorophores for ovarian cancer imaging, showing improved brightness, stability, and targeting across multiple tumor models.

Microphysiological Flow Batteries For Dynamic EDC Screening Of mESC-derived Thyroid Organoids

The paper introduces a thyroid-on-chip platform using mouse embryonic stem cell-derived thyroid organoids to dynamically screen endocrine disrupting chemicals (EDCs) and identify molecular signatures of thyroid disruption.