A. Upadhyaya

N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters

This study shows that N-WASP-dependent branched actin polymerization drives B-cell contraction, which reduces BCR signaling by increasing receptor cluster molecular density and displacing signaling molecules.

Three-dimensional structured illumination microscopy with enhanced axial resolution

The paper introduces two methods to enhance axial resolution in 3D SIM microscopy, achieving isotropic resolution with minimal hardware changes and using deep learning for improved imaging.

Single-molecule tracking reveals two low-mobility states for chromatin and transcriptional regulators within the nucleus

This study uses single-molecule tracking and machine learning to identify two low-mobility states of chromatin and transcriptional regulators, showing dynamic interactions and switching between states.

A tug of war between filament treadmilling and myosin induced contractility generates actin rings

The paper uses simulations and experiments to show that actin ring formation in cells depends on the balance between filament treadmilling and myosin contractility.

Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes

This paper applies RCAN to enhance fluorescence microscopy image volumes, achieving denoising and resolution improvements across multiple microscopy techniques.

Multiview super-resolution microscopy

This paper introduces a multiview super-resolution microscopy approach that enhances confocal microscopy resolution and reduces phototoxicity across multiple scales.

Comparative immunogenicity of bacterially expressed soluble trimers and nanoparticle displayed influenza hemagglutinin stem immunogens

This study compares the immunogenicity of soluble trimeric influenza stem domain immunogens and nanoparticle-displayed versions, showing similar protection against homologous and heterologous viral challenges.

Immunogenicity and protective efficacy of a highly thermotolerant, trimeric SARS-CoV-2 receptor binding domain derivative

This study designs a thermotolerant trimeric RBD vaccine candidate that elicits strong neutralizing antibodies against multiple SARS-CoV-2 variants in animal models.

Power-law behaviour of transcription factor dynamics at the single-molecule level implies a continuum affinity model

The paper analyzes single-molecule tracking data of transcription factors and finds power-law distributions, suggesting a continuum affinity model rather than discrete binding states.

Design of a highly thermotolerant, immunogenic SARS-CoV-2 spike fragment

This paper designs a thermotolerant, immunogenic SARS-CoV-2 RBD protein fragment that is stable at high temperatures and elicits neutralizing antibodies in guinea pigs.

Accelerating iterative deconvolution and multiview fusion by orders of magnitude

This paper introduces faster deconvolution and multiview fusion methods, achieving speedups of 10 to several thousand-fold over previous approaches.

N-WASP regulates the mobility of the B cell receptor and co-receptors during signaling activation

This study uses single molecule imaging and machine learning to show that N-WASP regulates BCR and co-receptor mobility during signaling, affecting actin dynamics and B cell responses.

Single-shot super-resolution total internal reflection fluorescence microscopy

This paper introduces a method combining instant structured illumination microscopy with TIRF to achieve higher resolution and faster imaging of live samples near the coverslip.

Reflective imaging improves resolution, speed, andcollection efficiency in light sheet microscopy

This paper introduces a reflective imaging technique that enhances resolution, speed, and collection efficiency in light sheet microscopy without altering the microscope hardware.