R. Varadarajan

Improved prediction of stabilizing mutations in proteins by incorporation of mutational effects on ligand binding

The paper improves stabilizing mutation prediction by combining computational methods with experimental data, achieving higher precision and thermal stability increases.

Enhanced protective efficacy of a novel, thermostable, RBD-S2 fusion immunogen against SARS-CoV-2 and its variants

This study evaluates a novel RBD-S2 fusion immunogen that shows enhanced protection against SARS-CoV-2 and its variants in animal models.

Mutations in S2 subunit of SARS-CoV-2 Omicron spike strongly influence its conformation, fusogenicity and neutralization sensitivity

This study investigates how mutations in the S2 subunit of SARS-CoV-2 Omicron spike affect its structure, fusogenicity, and neutralization sensitivity.

Mutational scan inferred binding energetics and structure in intrinsically disordered protein CcdA

The paper uses deep mutational scanning to study sequence-function relationships in the IDP CcdA, revealing insights into binding energetics and structural signatures.

High resolution cryo-EM structures of two potently SARS-CoV-2 neutralizing monoclonal antibodies of same donor origin that vary in neutralizing Omicron variants

The paper presents high-resolution cryo-EM structures of two SARS-CoV-2 neutralizing mAbs from the same donor, showing differences in their binding to Omicron variants and conformational changes in the spike protein.

Ter-Seq: A high-throughput method to stabilize transient ternary complexes and measure associated kinetics

The paper introduces Ter-Seq, a method to stabilize ternary complexes and measure kinetics using CcdA-CcdB-GyrA14 as a model, identifying mutations that affect complex formation and rejuvenation rates.

Highly Thermotolerant SARS-CoV-2 Vaccine Elicits Neutralising Antibodies Against Delta and Omicron in Mice

This study evaluates a highly thermotolerant SARS-CoV-2 vaccine in mice, showing it elicits neutralizing antibodies against Delta and Omicron variants.

Structural and functional determinants inferred from deep mutational scans

The paper uses deep mutational scans to identify and distinguish protein binding sites from buried and non-active residues using a toxin-antitoxin system.

Mechanistic insights into global suppressors of protein folding defects

The paper identifies global suppressor mutations that rescue protein folding defects and shows they stabilize proteins through refolding rate acceleration, with some providing structural insights.

Stabilizing proteins through saturation suppressor mutagenesis.

This paper introduces a method to identify stabilizing protein mutations by screening libraries with destabilizing mutations, demonstrating its effectiveness in enhancing protein stability.

Prediction of residue-specific contributions to binding and thermal stability using yeast surface display

The paper uses yeast surface display to predict residue-specific contributions to binding and thermal stability, showing high accuracy in identifying buried and active-site residues.

Comparative immunogenicity of bacterially expressed soluble trimers and nanoparticle displayed influenza hemagglutinin stem immunogens

This study compares the immunogenicity of soluble trimeric influenza stem domain immunogens and nanoparticle-displayed versions, showing similar protection against homologous and heterologous viral challenges.

Immunogenicity and protective efficacy of a highly thermotolerant, trimeric SARS-CoV-2 receptor binding domain derivative

This study designs a thermotolerant trimeric RBD vaccine candidate that elicits strong neutralizing antibodies against multiple SARS-CoV-2 variants in animal models.

Design of a highly thermotolerant, immunogenic SARS-CoV-2 spike fragment

This paper designs a thermotolerant, immunogenic SARS-CoV-2 RBD protein fragment that is stable at high temperatures and elicits neutralizing antibodies in guinea pigs.

Fascetto Interacting Protein (FIP) Regulates Fascetto (PRC1) to Ensure Proper Cytokinesis and Ploidy

This study identifies FIP as a regulator of Feo, showing that FIP is required for Feo localization and proper cytokinesis, with loss of both leading to cytokinesis defects and tumor-like masses.