J. Guan

Gradual labeling with fluorogenic probes for MINFLUX nanoscopy of densely packed structures

This paper introduces GLF-MINFLUX, a method for nanoscale imaging of densely packed structures, achieving improved localization precision and faster acquisition.

Defining the constituents and functions of a lipid-based jumbo phage compartment

The paper identifies a lipid-based compartment in early phage infection that protects phage DNA and supports early transcription and translation.

E3 ligase ASB3 downregulates antiviral innate immunity by targeting MAVS for ubiquitin-proteasomal degradation

This study identifies ASB3 as an E3 ligase that suppresses antiviral immunity by ubiquitinating and degrading MAVS, thereby inhibiting IFN-I responses and promoting viral replication.

AhR ligands from LGG metabolites promote piglet intestinal ILC3 activation and IL-22 secretion to inhibit PEDV infection

This study shows LGG metabolites activate ILC3 cells via AhR to produce IL-22, which prevents PEDV infection in piglets by enhancing intestinal cell proliferation and STAT3 signaling.

Attila and Aetius on the roof: The succession phenomenon in the roof greening area of a primary school in Beijing during the lockdown period of the COVID-19

The paper examines changes in rooftop green areas of a primary school in Beijing before and during the COVID-19 lockdown, focusing on invasive weed succession and its impact on cultivated plants.

An axonemal intron splicing program sustains Plasmodium male development

This study identifies RBPm1 as a splicing activator essential for axoneme assembly in Plasmodium male gametogenesis, revealing an intron splicing program critical for male development.

Predicting efficacy of immunotherapy in mice with triple negative breast cancer using a cholesterol PET radiotracer

The paper uses a cholesterol PET radiotracer to predict immunotherapy response in TNBC mouse models by measuring T cell cholesterol uptake.

ALK signalling primes the DNA damage response sensitizing ALK-driven neuroblastoma to ATR inhibition

This study identifies ALK signaling's role in enhancing DNA damage response in neuroblastoma, showing that combined ATR/ALK inhibition leads to sustained tumor response and differentiation.

Commensal Bacteria Maintain a Qa-1b-restricted Unconventional CD8+ T Population in Gut Epithelium

The paper identifies Qa-1b-restricted CD8+ T cells in the gut epithelium, showing they depend on commensal bacteria for maintenance.

The promiscuous development of an unconventional Qa1b-restricted T cell population

The paper identifies a CD8 T cell population, QFL T cells, restricted to MHC-E that develops independently of classical MHC I and requires Qa1b for agonist selection and functionality.

A family of novel immune systems targets early infection of nucleus-forming jumbo phages

This study identifies a novel immune system, Juk, in Pseudomonas aeruginosa that targets jumbo phages by inhibiting early phage transcription, DNA replication, and nucleus assembly.

Simple visualization of submicroscopic protein clusters with a phase-separation-based fluorescent reporter.

This paper introduces CluMPS, a fluorescent reporter that detects and amplifies submicroscopic protein clusters, enabling visualization of small oligomers and endogenous protein assemblies.

A Microfluidic Chip for LAMP-based Multiplex Detection of Pathogen

The paper develops a microfluidic chip for LAMP-based multiplex detection of three bacterial pathogens and reports LOD values for Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.

Bacteriophages antagonize cGAS-like immunity in bacteria

The paper identifies a phage-encoded antagonist of the bacterial CBASS immunity system, showing it is necessary for phage replication and demonstrating CBASS functions as a natural anti-phage immune system.

RNA targeting with CRISPR-Cas13a facilitates bacteriophage genome engineering

This paper develops a CRISPR-Cas13a-based method to edit bacteriophage genomes, enabling precise gene modifications and functional studies of phage genes.

Oncogenic protein condensates suppress growth factor perception and modulate drug tolerance

This study shows that oncogenic protein condensates suppress RTK signaling and modulate drug tolerance in ALK-driven cancers by sequestering adapter proteins and enabling paracrine survival signals.

Effects of Chronic Intermittent Hypoxia and Chronic Sleep Fragmentation on Gut Microbiome, Serum Metabolome, Liver and Adipose Tissue Morphology

This study examines how chronic intermittent hypoxia and chronic sleep fragmentation affect gut microbiota, serum metabolites, and tissue morphology in mice, revealing their impact on lipid metabolism.

Fam49b dampens TCR signal strength to regulate survival of positively selected thymocytes

This study shows that Fam49b suppresses TCR signaling to prevent excessive negative selection in thymocytes, ensuring normal T cell development.

A focal adhesion kinase-YAP signaling axis drives drug tolerant persister cells and residual disease in lung cancer

This study identifies a FAK-YAP signaling axis that drives drug-tolerant persister cells and residual disease in lung cancer, showing that inhibiting this axis improves tumor response to targeted therapies.

Characterization of morpho-functional traits in mesophotic corals reveals optimized light capture and photosynthesis

This study characterizes morpho-functional traits in mesophotic corals, finding that mesophotic morphotypes optimize light capture and photosynthesis under low-light conditions.

Effect of ORF7 of SARS-CoV-2 on the chemotaxis of monocytes and neutrophils in vitro

This study shows that SARS-CoV-2 ORF7 increases monocyte chemotaxis via CCL2 and decreases neutrophil recruitment via reduced IL-8.

LENG8 regulation of mRNA processing, is responsible for the control of mitochondrial activity

This study identifies LENG8 as an mRNA processing factor that regulates mitochondrial activity by controlling mRNA nuclear export and mitochondrial protein expression.

All-optical mechanobiology interrogation of YAP in human cancer and normal cells using a novel multi-functional system

This paper introduces an integrated system for automated multi-functional imaging and analysis to study YAP mechanosensitivity in human cells.

RTK-dependent inducible degradation of mutant PI3K alpha drives GDC-0077 (Inavolisib) efficacy

The paper shows that GDC-0077 and taselisib degrade mutant PI3K alpha via HER2-dependent mechanisms, enhancing efficacy in PIK3CA-mutant breast cancer.

Sustained Oxygenation Accelerates Diabetic Wound Healing by Simultaneously Promoting Epithelialization and Angiogenesis, and Decreasing Tissue Inflammation

This study shows sustained oxygenation improves diabetic wound healing by enhancing epithelialization, angiogenesis, and reducing inflammation without drugs.

Automatic Multi-functional Integration Program (AMFIP) towards All-optical Mechanobiology Interrogation

AMFIP enables automatic multi-functional live-cell imaging by integrating hardware and software without extra costs or hardware, demonstrating its utility in mechanobiology studies.

Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike

This study engineers a SARS-CoV-2 spike protein with a disulfide bond to stabilize the locked conformation, revealing structural dynamics and the role of domain D in conformational changes and viral fitness.

iRGD-liposomes enhance tumor delivery and therapeutic efficacy of antisense oligonucleotide drugs against primary prostate cancer and bone metastasis

This paper demonstrates that iRGD-liposomes enhance the delivery and efficacy of AR-ASO in treating prostate cancer and bone metastasis without increasing toxicity.

Exploring heteroaromatic rings as a replacement for the labile amide of antiplasmodial pantothenamides

This study replaces the amide group in pantothenamides with heteroaromatic rings to improve antiplasmodial activity against Plasmodium species.

SARS-Cov-2-, HIV-1-, Ebola-neutralizing and anti-PD1 clones are predisposed

The paper identifies public antibody clones in the human repertoire, including virus-neutralizing and anti-PD1 clones, and introduces a tool for analyzing Rep-seq data.