J. W. Taraska

Adhesion energy controls lipid binding-mediated endocytosis

The paper shows that multivalent lipid binding by globular particles induces membrane deformation and clathrin-independent endocytosis, with adhesion energy determining the threshold for internalization.

Local Monomer Levels and Established Filaments Potentiate Non-Muscle Myosin 2 Assembly

This study identifies biophysical mechanisms regulating non-muscle myosin 2 filament assembly using dynamic imaging and optogenetic tools, showing myosin monomer concentration and myosin:myosin interactions drive filament formation.

Three-dimensional structured illumination microscopy with enhanced axial resolution

The paper introduces two methods to enhance axial resolution in 3D SIM microscopy, achieving isotropic resolution with minimal hardware changes and using deep learning for improved imaging.

The molecular organization of flat and curved caveolae indicates bendable structural units at the plasma membrane.

This study identifies proteins involved in regulating caveolae shape using imaging techniques, revealing that dynamin is not directly involved in caveolae curvature.

A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells.

Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin mediated endocytosis

This study shows that sterols reduce the energy required for membrane bending and fission during clathrin-mediated endocytosis, and that their depletion impairs this process and affects cellular function.

Dual clathrin and adhesion signaling systems regulate growth factor receptor activation

The paper reveals how clathrin lattices and adhesion receptors interact to regulate growth factor signaling through coordinated crosstalk between EGFR and β5-integrin.

The nanoscale anatomy of exocytic dense-core vesicles in neuroendocrine cells

This study maps Rab-GTPases and their effectors at the nanoscale in exocytic dense-core vesicles using advanced imaging techniques and reveals their circumferential distribution on vesicles.

Structurally distinct endocytic pathways for B cell receptors in B lymphocytes

This study shows that B cells use different endocytic mechanisms depending on BCR cluster size, switching from clathrin-mediated endocytosis to large membrane invaginations with actin and FCHSD2 recruitment.

The structure and spontaneous curvature of clathrin lattices at the plasma membrane.

This study determines the structural framework of clathrin-mediated endocytosis, showing flat clathrin lattices spontaneously curve into coated pits without external factors.

Local Protein Dynamics During Microvesicle Exocytosis in Neuroendocrine Cells

The paper uses live-cell imaging to study protein dynamics during microvesicle exocytosis in neuroendocrine cells, revealing roles of endocytic proteins in regulating exocytosis.

Membrane bending begins at any stage of clathrin-coat assembly and defines endocytic dynamics

The paper uses advanced imaging techniques to study membrane bending during clathrin-coat assembly and finds that bending occurs at different stages of the process, challenging existing models.

Semisynthetic pH-Sensitive Fluorophores For Imaging Exocytosis And Endocytosis

This paper develops semisynthetic pH-sensitive fluorophores that enable imaging of exocytosis and endocytosis, matching the performance of GFP-based pHluorin.