A. Frost

Cardiolipin dynamics promote membrane remodeling by mitochondrial OPA1

This study investigates how cardiolipin dynamics influence mitochondrial membrane remodeling via OPA1 interactions using molecular dynamics and cryoEM.

Structural basis of TRPV1 modulation by endogenous bioactive lipids

This study identifies a lipid-binding pocket in TRPV1 and shows that its occupancy by phosphoinositides modulates channel activity, with lipid ejection triggering activation.

A structural biology community assessment of AlphaFold 2 applications

This study assesses AlphaFold 2's applications in structural biology, finding it outperforms existing tools in predicting disorder, complexes, and structural features with high confidence.

Brominated Lipid Probes Expose Structural Asymmetries in Constricted Membranes

The paper uses brominated lipids and cryo-EM to reveal structural asymmetries in constricted membranes.

Viral Evasion of the Integrated Stress Response Through Antagonistic eIF2-P Mimicry

This study shows that SFSV NSs protein evades the ISR by competing with eIF2-P for binding to eIF2B, preserving its activity and allowing viral protein synthesis.

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

This study uses directed evolution to identify Cfr variants that enhance rRNA methylation, revealing structural insights into antibiotic resistance mechanisms.

eIF2B Conformation and Assembly State Regulate the Integrated Stress Response

This study shows that eIF2B assembly and conformation regulate the ISR, with ISRIB affecting eIF2B through decamer stabilization and allosteric modulation.

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

This study optimizes K3 camera settings in CDS mode for cryo-EM, showing improved resolution and throughput for protein complex imaging.

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients

The paper reveals the structural and mechanistic basis of EMC's role in biogenesis of distinct transmembrane proteins through cryo-EM structures and functional studies.

Photocatalytic plant LPOR forms helical lattices that shape membranes for chlorophyll synthesis

This study reveals the atomic structure of LPOR assemblies and shows how they shape membranes and catalyze chlorophyll synthesis in plants.

Membrane constriction and thinning by sequential ESCRT-III polymerization

This study determines the structures of ESCRT-III filaments to show how sequential polymerization by CHMP1B and IST1 drives membrane tubulation, constriction, and thinning.

Anisotropic ESCRT-III architecture governs helical membrane tube formation

The paper describes how ESCRT-III filaments assemble into helical membrane tubes through anisotropic architecture and mathematical modeling.

High-resolution cryo-electron microscopy structure of the Escherichia coli 50S subunit and validation of nucleotide modifications

The paper presents a high-resolution cryo-EM structure of E. coli 50S subunit and develops a program for identifying rRNA modifications using cryo-EM maps.

The mechanisms of dynamin-actin interaction

This study identifies a noncanonical role for dynamin in cell-cell fusion by directly bundling actin filaments through GTPase-dependent interactions.

A role for liquid-liquid phase separation in ESCRT-mediated nuclear envelope reformation.

This study shows how LEM2 uses liquid-liquid phase separation and ESCRT pathways to facilitate nuclear envelope reformation during mitotic exit.

A tunable microfluidic device enables cargo encapsulation by cell- or organelle-sized lipid vesicles comprising asymmetric lipid monolayers

This paper introduces a microfluidic device for creating lipid vesicles with tunable sizes and asymmetric lipid monolayers, capable of encapsulating cargo and supporting membrane processes.

Structural basis of eIF2B-catalyzed GDP exchange and phosphoregulation by the integrated stress response

The paper reports cryoEM structures of eIF2 bound to eIF2B, revealing the mechanism of GDP exchange and phosphoregulation in the integrated stress response.

The ESCRT-III proteins IST1 and CHMP1B assemble around nucleic acids

The paper shows IST1 and CHMP1B form double-stranded filaments around nucleic acids, with structural features that suggest potential interactions with MIT domain-containing co-factors.

Vms1p is a release factor for the Ribosome-associated Quality control Complex

This study identifies Vms1p as the tRNA hydrolase that releases nascent polypeptides in the RQC pathway for degradation.

Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule

The paper determines the structure of eIF2B bound to ISRIB, revealing how it promotes eIF2B assembly and reverses eIF2 phosphorylation effects.

Structural Basis of Mitochondrial Receptor Binding and GTP Driven Conformational Constriction by Dynamin-Related Protein 1

This study presents cryoEM structures of DRP1 bound to MID49 and nucleotides, revealing how GTP binding drives conformational changes essential for mitochondrial fission.

LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells

This study identifies LEM2 and CHMP7 as conserved factors that recruit ESCRT-III proteins to the nuclear envelope during closure in fission yeast and human cells.