J. B. Huppa

TCR/CD3-based synthetic antigen receptors (TCC) convey superior antigen sensitivity combined with high fidelity of activation

This study shows TCR/CD3-based synthetic antigen receptors (TCC) have superior antigen sensitivity and activation fidelity compared to second-generation CARs.

Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion

The paper uses live cell microscopy to show that monomeric agonist pMHCIIs can activate T-cells autonomously without requiring synaptic engagement or bystander pMHCIIs.

3D single molecule localization microscopy reveals the topography of the immunological synapse at isotropic precision below 15 nm

This study uses 3D-SMLM to measure the cleft size in immunological synapses between T-cells and lipid bilayers, finding distances of 18-31 nm.

Strategies for the site-specific decoration of DNA origami nanostructures with functionally intact proteins

This study evaluates strategies for site-specific decoration of DNA origami with functional proteins, finding that PNA-based conjugation best preserves protein function and stoichiometry.

Temporal Analysis of T-Cell Receptor-Imposed Forces via Quantitative Single Molecule FRET Measurements

The paper measures T-cell receptor forces using single molecule FRET, finding a 5 pN force peak during T-cell activation on gel-phase SLBs.

DNA origami demonstrate the unique stimulatory power of single pMHCs as T-cell antigens

The paper demonstrates that single pMHCs can robustly stimulate T-cells as isolated entities, identifying the minimal requirements for effective TCR-triggering.

SARS-CoV-2 escapes CD8 T cell surveillance via mutations in MHC-I restricted epitopes

The paper identifies mutations in SARS-CoV-2 that reduce MHC-I binding and CD8+ T cell recognition, suggesting escape from immune surveillance.

The SPPL3-defined glycosphingolipid repertoire regulates immune responses by improving HLA class I access

The paper identifies a glycosphingolipid (GSL) signature that inhibits HLA-I antigen presentation and shows its reversal by clinically approved drugs.

Unscrambling Fluorophore Blinking for Comprehensive Cluster Detection via Photoactivated Localization Microscopy

The paper develops a method to quantify and correct for fluorophore blinking in PALM to improve cluster detection accuracy.

Inefficient ZAP70-Signaling Blunts Antigen Detection by CAR-T-Cells

This study identifies that inefficient ZAP70 signaling in CAR-T-cells reduces antigen detection sensitivity compared to TCRs.