J. B. Grimm

A series of spontaneously blinking dyes for super-resolution microscopy

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation

The paper shows that lysosomal amino acid release at ER junctions regulates translation of secretome mRNAs, with Lunapark and lysosomal function playing key roles.

Dynamic 1D Search and Processive Nucleosome Translocations by RSC and ISW2 Chromatin Remodelers

This study investigates the dynamic search and processive nucleosome translocation mechanisms of RSC and ISW2 chromatin remodelers using optical tweezers and single-particle imaging.

Optimizing multifunctional fluorescent ligands for intracellular labeling

The paper designs cell-permeable multifunctional ligands for HaloTag fusions that enable protein labeling and translocation, using Si-rhodamines with low KL-Z and high logD7.4 values.

Deuteration improves small-molecule fluorophores

This study shows that deuteration of small-molecule fluorophores enhances their brightness, chromostability, and photostability in cellular imaging.

Optimization and functionalization of red-shifted rhodamine dyes

This study optimizes red-shifted rhodamine dyes to improve their performance and enables functionalization with different chemical groups.

Multi-color single molecule imaging uncovers extensive heterogeneity in mRNA decoding

This study introduces MoonTag, a fluorescence labeling system to visualize single mRNA translation, revealing stochastic start site selection and dynamic regulation in real-time.

Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores

The paper introduces a bleaching-independent STED microscopy method using exchangeable fluorophores, enabling long-term whole-cell 3D and multi-color imaging in live cells.

Spot-On: robust model-based analysis of single-particle tracking experiments

Spot-On provides a bias-corrected analysis tool for single-particle tracking experiments, outperforming existing methods and demonstrating robust inference of diffusion constants and subpopulations.

A general method to fine-tune fluorophores for live-cell and in vivo imaging

The paper introduces a method to fine-tune fluorophores for improved brightness and photostability in live-cell and in vivo imaging.

Semisynthetic pH-Sensitive Fluorophores For Imaging Exocytosis And Endocytosis

This paper develops semisynthetic pH-sensitive fluorophores that enable imaging of exocytosis and endocytosis, matching the performance of GFP-based pHluorin.