L. D. Lavis

A series of spontaneously blinking dyes for super-resolution microscopy

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

Transforming chemigenetic bimolecular fluorescence complementation systems into chemical dimerizers using chemistry

The paper develops novel fluorogens that activate FAST proteins and convert splitFAST systems into chemical dimerizers, reducing the need for protein engineering.

Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment

The paper uses correlative single molecule lattice light sheet imaging to study nucleosome dynamics and chromatin environment interactions in live cells, revealing differential diffusion and packing with increasing chromatin density.

Dynamic 1D Search and Processive Nucleosome Translocations by RSC and ISW2 Chromatin Remodelers

This study investigates the dynamic search and processive nucleosome translocation mechanisms of RSC and ISW2 chromatin remodelers using optical tweezers and single-particle imaging.

Optimizing multifunctional fluorescent ligands for intracellular labeling

The paper designs cell-permeable multifunctional ligands for HaloTag fusions that enable protein labeling and translocation, using Si-rhodamines with low KL-Z and high logD7.4 values.

Bromodomains regulate dynamic targeting of the PBAF chromatin remodeling complex to chromatin hubs

This study uses live-cell imaging to show that PBAF chromatin remodeler dynamically targets acetylated chromatin hubs via bromodomains, with distinct binding kinetics in euchromatin and heterochromatin.

Deuteration improves small-molecule fluorophores

This study shows that deuteration of small-molecule fluorophores enhances their brightness, chromostability, and photostability in cellular imaging.

Optimization and functionalization of red-shifted rhodamine dyes

This study optimizes red-shifted rhodamine dyes to improve their performance and enables functionalization with different chemical groups.

Multi-color single molecule imaging uncovers extensive heterogeneity in mRNA decoding

This study introduces MoonTag, a fluorescence labeling system to visualize single mRNA translation, revealing stochastic start site selection and dynamic regulation in real-time.

Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores

The paper introduces a bleaching-independent STED microscopy method using exchangeable fluorophores, enabling long-term whole-cell 3D and multi-color imaging in live cells.

Spot-On: robust model-based analysis of single-particle tracking experiments

Spot-On provides a bias-corrected analysis tool for single-particle tracking experiments, outperforming existing methods and demonstrating robust inference of diffusion constants and subpopulations.

Visualizing Long-Term Single-Molecule Dynamics in vivo by Stochastic Protein Labeling

This paper introduces a genetic strategy for sparse protein labeling in live cells, enabling long-term single-molecule tracking and revealing new insights into molecular dynamics in vivo.

Nuclear Microenvironments Modulate Transcription From Low-Affinity Enhancers

The paper shows that low-affinity enhancer binding by Ubx is efficient in nuclear microenvironments with high Ubx concentration, aided by cofactor Hth.

A general method to fine-tune fluorophores for live-cell and in vivo imaging

The paper introduces a method to fine-tune fluorophores for improved brightness and photostability in live-cell and in vivo imaging.

Semisynthetic pH-Sensitive Fluorophores For Imaging Exocytosis And Endocytosis

This paper develops semisynthetic pH-sensitive fluorophores that enable imaging of exocytosis and endocytosis, matching the performance of GFP-based pHluorin.