Author

L. Shrestha

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Microbiology· 2 Biophysics· 1 Evolutionary Biology· 1

Signature Assays, Organisms & Methods

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Organism:human· 7 Approach:experimental assay· 6 Assay:structural biology· 4 Research Focus:protein structure prediction· 3 Assay:bulk RNA-seq· 2 Research Focus:host-pathogen & immune response· 2 Research Focus:protein folding & dynamics· 2 Research Focus:cell type annotation· 1

Frequent Co-Authors

E. P. Carpenter· 4 shared N. A. Burgess-Brown· 4 shared S. R. Bushell· 4 shared A. Chu· 3 shared A. L. Greninger· 3 shared H. Xie· 3 shared K. R. Jerome· 3 shared P. Roychoudhury· 3 shared

Activity by Year

2018-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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4 shared papersBiochemistry

E. P. Carpenter

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Author profile →

8 papers

Recent·Most cited

Structure and function of the SIT1 proline transporter in complex with the COVID-19 receptor ACE2

H. Z. Li, A. C. W. Pike, G. Chi, J. S. Hansen, S. G. Lee, K. E. J. Rodstrom, S. R. Bushell, D. Speedman, A. Evans, D. Wang, D. He, L. Shrestha, C. Nasrallah, N. A. Burgess-Brown, T. R. Dafforn, , D. B. Sauer

The paper determines the structure of the SIT1 proline transporter complexed with ACE2 using cryo-EM, revealing substrate specificity and structural changes related to transport and a disease-causing mutation.

bioRxiv · May 17, 2023Biophysics

Narrow transmission bottlenecks and limited within-host viral diversity during a SARS-CoV-2 outbreak on a fishing boat

W. H. Hannon, P. Roychoudhury, H. Xie, L. Shrestha, A. Addetia, K. R. Jerome, A. L. Greninger, J. D. Bloom

This study investigates SARS-CoV-2 transmission on a fishing boat, finding low within-host viral diversity and limited viral evolution during the outbreak.

bioRxiv · Feb 9, 2022Evolutionary Biology

Molecular basis for the regulation of human glycogen synthase by phosphorylation and glucose-6-phosphate

T. J. McCorvie, P. M. Loria, M. Tu, S. Han, L. Shrestha, D. S. Froese, I. M. Ferreira, A. P. Berg, W. W. Yue

This study presents cryo-EM structures of phosphorylated human GYS1 complexed with GYG1, revealing how phosphorylation and glucose-6-phosphate regulate glycogen synthase activity.

bioRxiv · Nov 12, 2021Biochemistry

The structural basis of fatty acid elongation by the ELOVL elongases

L. Nie, A. C. Pike, T. C. Pascoa, S. R. Bushell, A. Quigley, G. F. Ruda, A. Chu, V. Cole, D. Speedman, T. Moreira, L. Shrestha, S. M. Mukhopadhyay, N. A. Burgess-Brown, J. D. Love, P. E. Brennan, E. P. Carpenter

The paper reports the structure of the human ELOVL7 elongase, revealing its catalytic mechanism and substrate-binding sites, with implications for inhibiting VLCFA accumulation in diseases.

bioRxiv · Nov 11, 2020Biochemistry

In vivo antiviral host response to SARS-CoV-2 by viral load, sex, and age

N. A. P. Lieberman, V. Peddu, H. Xie, L. Shrestha, M. Huang, M. C. Mears, M. N. Cajimat, D. A. Bente, P.-Y. Shi, F. Bovier, P. Roychoudhury, K. R. Jerome, A. Moscona, M. Porotto, A. L. Greninger

This study examines how SARS-CoV-2 induces antiviral host responses, showing differences by viral load, age, and sex in gene expression profiles from infected individuals.

PLOS Biology · Sep 8, 2020Peer-reviewedMicrobiology

SARS-CoV-2 ORF6 disrupts nucleocytoplasmic transport through interactions with Rae1 and Nup98

A. Addetia, N. A. P. Lieberman, Q. Phung, H. Xie, P. Roychoudhury, M. A. Loprieno, M. Huang, L. Shrestha, K. R. Jerome, A. L. Greninger

This paper shows SARS-CoV-2 ORF6 interacts with Rae1 and Nup98 to disrupt nucleocytoplasmic transport, reducing reporter expression and causing nuclear mRNA accumulation, more strongly than SARS-CoV ORF6.

bioRxiv · Aug 3, 2020Microbiology

The structural basis of lipid scrambling and inactivation in the endoplasmic reticulum scramblase TMEM16K

S. R. Bushell, A. C. W. Pike, M. E. Falzone, N. J. G. Rorsman, C. M. Ta, R. A. Corey, T. D. Newport, C. A. Shintre, A. Tessitore, A. Chu, Q. Wang, L. Shrestha, S. Mukhopadhyay, J. D. Love, N. A. Burgess-Brown, , E. P. Carpenter

This study identifies TMEM16K as an ER-resident calcium-regulated lipid scramblase and elucidates its structural basis for lipid scrambling and inactivation.

bioRxiv · Dec 12, 2018Biochemistry

Structures of DPAGT1 explain glycosylation disease mechanisms and advance TB antibiotic design

Y. Y. Dong, H. Wang, A. C. Pike, S. J. Cochrane, S. Hamedzade, F. J. Wyszynsk, S. R. Bushell, S. F. Royer, D. A. Widdick, A. Sajid, H. I. Boshoff, R. Lucas, W.-M. Liu, S. S. Lee, T. Machida, , E. P. Carpenter

This paper determines the structures of DPAGT1 with substrates and inhibitors, revealing catalytic mechanisms and disease-causing mutations, and designs non-toxic antibiotics for tuberculosis.

bioRxiv · Apr 2, 2018Biochemistry