C. M. Dobson

Parallel and sequential pathways of molecular recognition of a tandem-repeat protein and its intrinsically disordered binding partner

This study investigates the kinetic mechanisms of TCF7L2 binding to β-catenin, revealing a two-site avidity model with parallel dissociation pathways.

Machine learning aided top-down protein identification on a microfluidic platform

This paper demonstrates a microfluidic platform that generates multidimensional protein fingerprints, enabling high-accuracy classification using machine learning, even when single-dimensional classification fails.

The Hsc70 Disaggregation Machinery RemovesMonomer Units Directly from α-Synuclein FibrilEnds

This study shows the Hsc70-DnaJB1-Apg2 chaperone system removes monomer units directly from α-synuclein fibril ends, reversing aggregation without intermediate fragmentation.

The extent of protein hydration dictates the preference for heterogeneous or homogeneous nucleation generating either parallel or antiparallel β-sheet α-synuclein aggregates

This study shows that α-synuclein can form amyloid aggregates via homogeneous nucleation under limited hydration, leading to antiparallel β-sheet structures, which are more stable than parallel ones under such conditions.

Small molecule sequestration of amyloid-β as a drug discovery strategy for Alzheimer's disease

This paper identifies a small molecule that binds and sequesters monomeric amyloid-β, inhibiting its aggregation and rescuing toxicity in a C. elegans model.

Microfluidic Antibody Affinity Profiling for In-Solution Characterisation of Alloantibody - HLA Interactions in Human Serum

This paper develops a microfluidic assay to directly measure antibody affinity and concentration in solution, applied to characterise alloantibody-HLA interactions in transplant patient sera.

Structural characterization of covalently stabilized human cystatin C oligomers

The paper characterizes the structure of covalently stabilized human cystatin C oligomers using molecular modeling and experimental techniques, revealing a dodecamer model with specific dimensions.

Dynamics of oligomer populations formed during the aggregation of Alzheimer's Aβ42 peptide

This study investigates the dynamics of Aβ42 oligomers during amyloid fibril formation, revealing that most oligomers dissociate into monomers rather than forming fibrils.

Rapid Fractionation and Characterisation of Alpha-Synuclein Oligomers in Solution

This study demonstrates microfluidic free-flow electrophoresis for rapidly separating and characterizing alpha-synuclein oligomers in solution.

Kinetic fingerprints differentiate anti-Aβ therapies

This study uses kinetic analysis and binding measurements to compare four anti-Aβ antibodies, showing aducanumab uniquely reduces Aβ oligomer flux.

Thermodynamic and kinetic design principles for protein aggregation inhibitors

This study develops a kinetic theory to identify thermodynamic and kinetic signatures of effective protein aggregation inhibitors.

Escalating protein supersaturation underlies inclusion formation in muscle proteinopathies

The paper quantifies protein supersaturation in muscle biopsies from PAM patients, showing it escalates near pathological inclusions and correlates with aggregation.

ThX - A next-generation probe for the early detection of amyloid aggregates

This paper introduces ThX, a new fluorescent probe for detecting amyloid aggregates, showing improved brightness and binding affinity over ThT.

An engineered monomer binding-protein for α-synucleinefficiently inhibits the proliferation of amyloid fibrils

The paper engineers a monomer-binding protein that inhibits α-synuclein fibril proliferation in vitro and in vivo, showing it reduces aggregates and related pathologies.

Fast fluorescence lifetime imaging reveals the aggregation processes of α-synuclein and polyglutamine in aging Caenorhabditis elegans

This study uses fast TG-FLIM to analyze α-synuclein and polyglutamine aggregation in aging C. elegans, revealing differences in their aggregation kinetics and amyloid-like features.