Author

M. P. Rout

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Cell Biology· 4 Biophysics· 3

Signature Assays, Organisms & Methods

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Approach:experimental assay· 9 Research Focus:chromatin & epigenetic regulation· 4 Research Focus:cell type annotation· 3 Organism:yeast· 3 Research Focus:drug response / drug discovery· 2 Organism:human· 2 Research Focus:protein folding & dynamics· 2 Assay:proteomics· 2

Frequent Co-Authors

B. T. Chait· 8 shared A. Sali· 6 shared F. D. Mast· 3 shared N. E. Ketaren· 3 shared P. C. Fridy· 3 shared T. Sanyal· 3 shared B. Raveh· 2 shared D. Singh· 2 shared

Activity by Year

2019-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 30 mutual co-author links

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8 shared papersBiochemistry

B. T. Chait

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Author profile →

11 papers

Recent·Most cited

The Molecular Architecture of the Nuclear Basket

D. Singh, N. Soni, J. Hutchings, I. Echeverria, F. Shaikh, M. Duquette, S. Suslov, Z. Li, T. van Eeuwen, K. Molloy, Y. Shi, J. Wang, Q. Guo, B. T. Chait, J. Fernandez-Martinez, , E. Villa

This study uses structural biology to reveal the architecture of the nuclear basket in yeast and mammals, showing how Mlp/Tpr proteins form its structural components.

bioRxiv · Mar 27, 2024Biophysics

Integrative mapping reveals molecular features underlying the mechanism of nucleocytoplasmic transport

B. Raveh, R. Eliasian, S. Rashkovits, D. Russel, R. Hayama, S. Sparks, D. Singh, R. Lim, E. Villa, M. P. Rout, D. Cowburn, A. Sali

The paper constructs an integrative Brownian dynamics model of nucleocytoplasmic transport, revealing how FG repeat proteins and nuclear transport receptors mediate selective transport through NPCs.

bioRxiv · Dec 31, 2023 · PreprintCell Biology

Unique Binding and Stabilization Mechanisms Employed By and Engineered Into Nanobodies.

N. E. Ketaren, P. C. Fridy, V. Malashkevich, T. Sanyal, M. Brilliantes, M. K. Thompson, D. A. Orens, J. B. Bonanno, a. Sali, S. C. Almo, B. T. Chait, M. P. Rout

This study identifies mechanisms enabling nanobodies to bind antigens with high affinity and stability, revealing a conserved region critical for their function and suggesting strategies for their optimization.

bioRxiv · Oct 22, 2023 · PreprintBiochemistry

Nanobody repertoire generated against the spike protein of ancestral SARS-CoV-2 remains efficacious against the rapidly evolving virus

N. E. Ketaren, F. D. Mast, P. C. Fridy, J. P. Olivier, T. Sanyal, A. Sali, B. T. Chait, M. P. Rout, J. Aitchison

The paper shows that nanobodies against ancestral SARS-CoV-2 spike protein remain effective against recent variants, including omicron sublineages and emerging XBB/BQ.1.1 variants.

bioRxiv · Jul 14, 2023Biochemistry

Dynamic molecular mechanism of the nuclear pore complex permeability barrier

T. Kozai, J. Fernandez-Martinez, T. van Eeuwen, P. Gallardo, L. E. Kapinos, A. Mazur, W. Zhang, J. Tempkin, R. Panatala, M. Delgado-Izquierdo, B. Raveh, A. Sali, B. T. Chait, L. M. Veenhoff, M. P. Rout, R. Y. H. Lim

The paper investigates the dynamic behavior of the nuclear pore complex permeability barrier in yeast, showing it functions as a dynamic polymer brush rather than a phase-separated condensate.

bioRxiv · Mar 31, 2023 · PreprintBiophysics

Mex67 paralogs mediate division of labor in trypanosome RNA processing and export.

S. O. Obado, M. Stein, E. Hegedusova, W. Zhang, S. Hutchinson, L. Glover, Z. Paris, B. T. Chait, M. C. Field, M. P. Rout

This study identifies distinct roles for three Mex67 paralogs in trypanosome RNA processing and export, showing non-redundant functions in mRNA export and ribosome biogenesis.

bioRxiv · Jun 27, 2022 · PreprintCell Biology

The nucleoporin Nup170 mediates subtelomeric gene silencing through the Ctf18-RFC complex and PCNA

S. Kumar, M. L. Neal, S. Li, A. T. Navare, F. D. Mast, M. P. Rout, J. D. Aitchison

This study identifies Nup170's role in subtelomeric gene silencing by regulating PCNA levels through the Ctf18-RFC complex and Mlp1/Mlp2-dependent NPC subpopulations.

bioRxiv · Jun 17, 2022 · PreprintCell Biology

Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2

F. D. Mast, P. C. Fridy, N. E. Ketaren, J. Wang, E. Y. Jacobs, J. P. Olivier, T. Sanyal, K. R. Molloy, F. Schmidt, M. Rutkowska, Y. Weisblum, L. M. Rich, E. R. Vanderwall, N. Dambrauskas, V. Vigdorovich, , M. P. Rout

This paper presents a large repertoire of high-affinity nanobodies against SARS-CoV-2 Spike protein with potent neutralization properties and resistance to viral variants.

bioRxiv · Apr 8, 2021Biochemistry

Heh2/Man1 may be an evolutionarily conserved sensor of NPC assembly state

S. Borah, D. J. Thaller, Z. Hakhverdyan, E. C. Rodriguez, M. P. Rout, M. C. King, C. P. Lusk

The paper shows Heh2/Man1 interacts with NPC components in yeast and suggests it senses NPC assembly state for quality control and segregation.

bioRxiv · Jun 29, 2020Cell Biology

Affinity proteomic dissection of the human nuclear cap-binding-complex interactome

Y. Dou, S. Kalmykova, M. Pashkova, M. Oghbaie, H. Jiang, K. R. Molloy, B. T. Chait, M. P. Rout, D. Fenyo, T. H. Jensen, I. Altukhov, J. LaCava

This study maps the human nuclear cap-binding complex interactome using affinity proteomics to clarify the composition and interactions of NCBP1, NCBP2, and NCBP3-related macromolecular assemblies.

bioRxiv · Apr 20, 2020Biochemistry

Interactions of nuclear transport factors and surface-conjugated FG nucleoporins: Insights and limitations

R. Hayama, M. Sorci, J. J. Keating, L. M. Hecht, J. L. Plawsky, G. Belfort, B. T. Chait, M. P. Rout

The paper evaluates three surface-based methods for characterizing protein-protein interactions involving intrinsically disordered proteins, revealing previously overlooked mass transport limitations and additional binding modes.

bioRxiv · Mar 19, 2019Biophysics