P. Fromme

Minimized Sample Consumption for Time-Resolved Serial Crystallography Applied to the Redox Cycle of Human NQO1

This paper demonstrates a method to reduce sample consumption in time-resolved serial crystallography by using segmented droplet generation and mix-and-inject approaches for studying NQO1's redox cycle.

Detection of a geminate photoproduct of bovine cytochrome c oxidase by time-resolved serial femtosecond crystallography

The paper detects a geminate photoproduct of bovine cytochrome c oxidase using time-resolved serial femtosecond crystallography, revealing the ligand dissociation trajectory and protein dynamics.

Structural and biophysical properties of FopA, a major outer membrane protein of Francisella tularensis

This study determines the structural and biophysical properties of FopA, a major outer membrane protein of Francisella tularensis, revealing its dimeric, two-domain organization.

Characterization and computational simulation of human Syx, a RhoGEF implicated in glioblastoma

This study characterizes human Syx, a RhoGEF involved in glioblastoma, and performs computational simulations to elucidate its structure and allosteric regulation.

New Structural Insights into the Function of the Catalytically Active Human Taspase1

This study determines the crystal structure of catalytically active human Taspase1 and identifies a long helical domain essential for its enzymatic activity, suggesting it as a potential target for anticancer therapeutics.

Direct Observation of the Mechanism of Antibiotic Resistance by Mix-and-Inject at the European XFEL

The paper directly observes fast ligand binding in a biomedically relevant enzyme using MHz X-ray pulses at the European XFEL.

CryoFold: Ab-initio structure determination from electron density maps using molecular dynamics

CryoFold determines protein structures from electron density maps using molecular dynamics and physical knowledge, producing ensembles of low-energy conformations validated experimentally.

Enzyme Intermediates Captured "on-the-fly" by Mix-and-Inject Serial Crystallography

This paper demonstrates a method to capture enzyme reactions in real-time using mix-and-inject serial crystallography, revealing antibiotic cleavage and kinetic transitions in Mycobacterium tuberculosis β-lactamase.