R. D. Vale

De novo protein identification in mammalian sperm using high-resolution in situ cryo-electron tomography

The paper identifies novel sperm proteins in mammals using cryo-electron tomography and AlphaFold2 models, revealing their structural roles in flagellar mechanics.

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

This study uses cryo-electron tomography to reveal the asymmetric protein complexes in mammalian sperm axonemes, explaining their unique flagellar beating pattern.

Mechanical stretch regulates macropinocytosis in Hydra vulgaris

The paper investigates macropinocytosis in Hydra vulgaris, showing that mechanical stretch inhibits the process via calcium influx from stretch-activated channels.

Tight nanoscale clustering of Fc gamma-receptors using DNA origami promotes phagocytosis

The paper shows that tightly clustering Fc gamma-receptors using DNA origami enhances phagocytosis by increasing receptor phosphorylation and downstream signaling efficiency.

DNA origami patterning of synthetic T cell receptors reveals spatial control of the sensitivity and kinetics of signal activation

The paper uses DNA origami to pattern synthetic T cell receptors and shows how spatial arrangement affects signaling sensitivity and kinetics.

Three-color single-molecule imaging reveals conformational dynamics of dynein undergoing motility

This study uses three-color single-molecule imaging to track dynein's conformational dynamics during motility, revealing variable stepping between domains and a large number of conformational states.

A 6-nm ultra-photostable DNA Fluorocube for fluorescence imaging

The paper develops a DNA Fluorocube with enhanced photostability for fluorescence imaging, enabling precise tracking of motor proteins.

Rewired signaling network in T cells expressing the chimeric antigen receptor (CAR)

This study shows CAR T cells bypass LAT for some signaling outputs but still require it for IL-2 production, revealing a rewired signaling pathway.

CD47 suppresses phagocytosis by repositioning SIRPA and preventing integrin activation

This study reveals that CD47 suppresses phagocytosis by repositioning SIRPA and preventing integrin activation, offering new strategies for cancer immunotherapy.

Coupling of ATPase activity, microtubule binding and mechanics in the dynein motor domain

This study identifies mutations in dynein's stalk that disrupt directional motion and reveal the role of the stalk in coupling conformational changes across the AAA ring.

A Composition-Dependent Molecular Clutch Between T Cell Signaling Clusters and Actin

The paper investigates how Nck and WASP mediate the movement of LAT condensates across actin networks during T cell activation.

Chimeric antigen receptors that trigger phagocytosis

This paper introduces CAR-Ps, chimeric antigen receptors that direct macrophages to engulf specific targets like cancer cells, showing over 40% reduction in cancer cell numbers in co-culture.

Insights into centriole biogenesis and evolution revealed by cryoTomography of doublet and triplet centrioles.

This study uses cryo-tomography to compare mammalian and Drosophila centrioles, revealing structural differences and conserved features in their core regions.

Spatial Control of Draper Receptor Signaling Initiates Apoptotic Cell Engulfment

This study shows that Draper receptor signaling, similar to TCR, initiates engulfment of apoptotic cells through microcluster formation and actin depolymerization.

The actin cytoskeleton governs apical mitosis and daughter cell dispersion in intestinal epithelia

The paper investigates how actin cytoskeleton rearrangements during mitosis in intestinal epithelial cells lead to apical DNA positioning and daughter cell dispersion.

RNA Gelation in Repeat Expansion Disorders

This study shows that RNA repeat expansions can undergo gelation, forming clusters that resemble disease-associated nuclear foci, and that this gelation occurs above a critical repeat threshold similar to disease manifestation.

T cell co-stimulatory receptor CD28 is a primary target for PD-1-mediated inhibition

This study shows PD-1 inhibits T cell activation by preferentially dephosphorylating CD28 over TCR, revealing CD28 as a primary target for PD-1-mediated suppression.

A DNA-based T cell receptor reveals a role for receptor clustering in ligand discrimination

This study uses DNA-based T cell receptors to show that receptor clustering enhances ligand discrimination by enabling kinetic proofreading.