D. A. Agard

Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking

Cryo-EM reveals how Hsp90 and FKBP immunophilins co-regulate the Glucocorticoid Receptor

This paper presents cryo-EM structures of GR:Hsp90:FKBP51 and GR:Hsp90:FKBP52 complexes, revealing their distinct roles in regulating GR activity through different binding interfaces and allosteric mechanisms.

De novo protein identification in mammalian sperm using high-resolution in situ cryo-electron tomography

The paper identifies novel sperm proteins in mammals using cryo-electron tomography and AlphaFold2 models, revealing their structural roles in flagellar mechanics.

A family of novel immune systems targets early infection of nucleus-forming jumbo phages

This study identifies a novel immune system, Juk, in Pseudomonas aeruginosa that targets jumbo phages by inhibiting early phage transcription, DNA replication, and nucleus assembly.

Hsp90 provides a platform for kinase dephosphorylation by PP5

The paper shows Hsp90 facilitates PP5-mediated dephosphorylation of CRaf by scaffolding PP5's interaction with Hsp90-bound CRaf, revealing a new role for Hsp90 in post-translational modification of client kinases.

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

This study uses cryo-electron tomography to reveal the asymmetric protein complexes in mammalian sperm axonemes, explaining their unique flagellar beating pattern.

The {varphi}PA3 Phage Nucleus is Enclosed by a Self-Assembling, 2D Crystalline Lattice

The paper shows that the Phage Nucleus of {phi}PA3 forms a self-assembling, 2D crystalline lattice with adaptable inter-tetramer contacts and functional channels.

Structures of the active HER2/HER3 receptor complex reveal dynamics at the dimerization interface induced by binding of a single ligand

This study presents cryo-EM structures of the HER2/HER3 receptor complex bound to NRG1β, revealing conformational dynamics at the dimerization interface and how mutations and therapeutics interact with this interface.

Entropy Regularized Deconvolution of Cellular Cryo-Transmission Electron Tomograms

The paper applies entropy regularized deconvolution to cryo-ET data to improve resolution and reduce noise, assessing results via Fourier analysis and subtomogram analysis.

CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleation

This study elucidates the role of the CM1 motif in Spc110p in facilitating γ-Tubulin Small Complex (γTuSC) assembly and γ-Tubulin Ring Complex (γTuRC) formation in yeast.

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

This study optimizes K3 camera settings in CDS mode for cryo-EM, showing improved resolution and throughput for protein complex imaging.

GR chaperone cycle mechanism revealed by cryo-EM: inactivation of GR by GR:Hsp90:Hsp70:Hop client-loading complex

The paper reports the cryo-EM structure of the GR loading complex, revealing the molecular mechanism of GR inactivation and chaperone coordination.

Cryo-EM analysis of human mitochondrial Hsp90 in multiple tetrameric states

This study uses cryo-EM to reveal multiple tetrameric states of human TRAP1, showing dimer-dimer interactions and domain swapping contribute to tetramer stability.

GR chaperone cycle mechanism revealed by cryo-EM: reactivation of GR by the GR:Hsp90:p23 client-maturation complex

This paper reveals the molecular mechanism of GR activation by Hsp90 through cryo-EM, showing GR ligand binding is restored and stabilized by the Hsp90:p23 complex.

Microtubules form by progressively faster tubulin accretion, not by nucleation-elongation

The paper proposes a new model for microtubule formation that explains their assembly through accretion and straightening penalties, fitting experimental data better than traditional nucleation-elongation models.

A molecular pore spans the double membrane of the coronavirus replication organelle

The paper identifies a molecular pore in coronavirus replication organelles that facilitates RNA export to the cytosol, suggesting it as a potential drug target.

Modulation of mitochondrial Hsp90 (TRAP1) ATPase activity by calcium and magnesium

This study shows calcium can replace magnesium to support TRAP1 ATPase activity, leading to cooperative ATP hydrolysis and a mechanism for controlling TRAP1 function based on ATP and divalent cation concentrations.

Insights into centriole biogenesis and evolution revealed by cryoTomography of doublet and triplet centrioles.

This study uses cryo-tomography to compare mammalian and Drosophila centrioles, revealing structural differences and conserved features in their core regions.

A simple and robust procedure for preparing graphene-oxide cryo-EM grids

This paper describes a method for preparing graphene-oxide cryo-EM grids that are suitable for high-resolution single particle cryo-EM.

The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles

This study identifies the CM2 domain of centrosomin as a multi-functional binding domain with distinct roles in cell cycle-dependent centrosome organization.

Structural Basis of Mitochondrial Receptor Binding and GTP Driven Conformational Constriction by Dynamin-Related Protein 1

This study presents cryoEM structures of DRP1 bound to MID49 and nucleotides, revealing how GTP binding drives conformational changes essential for mitochondrial fission.